000038404 001__ 38404
000038404 005__ 20190625111330.0
000038404 0247_ $$2pmid$$apmid:15213973
000038404 0247_ $$2DOI$$a10.1002/elps.200305833
000038404 0247_ $$2WOS$$aWOS:000222396000005
000038404 0247_ $$2altmetric$$aaltmetric:6147073
000038404 037__ $$aPreJuSER-38404
000038404 041__ $$aeng
000038404 082__ $$a570
000038404 084__ $$2WoS$$aBiochemical Research Methods
000038404 084__ $$2WoS$$aChemistry, Analytical
000038404 1001_ $$0P:(DE-HGF)0$$aWeber, G.$$b0
000038404 245__ $$aImproved separation of palladium species in  biological matrices by using a combination of gel  permeation chromatography and isotachophoresis
000038404 260__ $$aWeinheim$$bWiley-Blackwell$$c2004
000038404 300__ $$a1758 - 1764
000038404 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000038404 3367_ $$2DataCite$$aOutput Types/Journal article
000038404 3367_ $$00$$2EndNote$$aJournal Article
000038404 3367_ $$2BibTeX$$aARTICLE
000038404 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000038404 3367_ $$2DRIVER$$aarticle
000038404 440_0 $$01792$$aElectrophoresis$$v25$$x0173-0835
000038404 500__ $$aRecord converted from VDB: 12.11.2012
000038404 520__ $$aThe binding of palladium to high-molecular-mass compounds in palladium-treated lettuce is investigated as an example for a biological matrix. The total palladium concentration in lettuce leaves is 10.3 ng/g wet weight. After homogenization, high-molecular-mass compounds (> 10 kDa) are isolated by ultrafiltration. For separation of these palladium species a combination of preparative gel permeation chromatography (GPC) and preparative isotachophoresis (ITP) is used. Palladium is determined in separated fractions by using a highly sensitive total reflection X-ray fluorescence (TXRF) method after preconcentration. After GPC separation, four main fractions of palladium species are collected, each containing palladium in ng quantities (3-10 ng). Two of these fractions are further separated by ITP, yielding at least three main peaks per GPC fraction, each containing palladium in the range of 0.3-3 ng. These palladium containing peaks are characterized by high-performance size exclusion chromatography (HPSEC) and capillary isotachophoresis (cITP) in parallel. HPSEC enables the estimation of the molecular mass of six main palladium peaks, covering a molecular mass range of 69-200 kDa. It is also shown that the estimation of molecular mass after separation is more reliable than the respective estimation directly in the first GPC run. However, cITP reveals that each of the separated peaks is still a mixture of at least five different compounds.
000038404 536__ $$0G:(DE-Juel1)FUEK257$$2G:(DE-HGF)$$aChemie und Dynamik der Geo-Biosphäre$$cU01$$x0
000038404 588__ $$aDataset connected to Web of Science, Pubmed
000038404 650_2 $$2MeSH$$aChromatography, Gel
000038404 650_2 $$2MeSH$$aElectrophoresis
000038404 650_2 $$2MeSH$$aFluorescence
000038404 650_2 $$2MeSH$$aLettuce: chemistry
000038404 650_2 $$2MeSH$$aPalladium: chemistry
000038404 650_2 $$2MeSH$$aPlant Leaves: chemistry
000038404 650_2 $$2MeSH$$aUltrafiltration
000038404 650_2 $$2MeSH$$aX-Rays
000038404 650_7 $$07440-05-3$$2NLM Chemicals$$aPalladium
000038404 650_7 $$2WoSType$$aJ
000038404 65320 $$2Author$$agel permeation chromatography
000038404 65320 $$2Author$$aisotachophoresis
000038404 65320 $$2Author$$apalladium
000038404 7001_ $$0P:(DE-HGF)0$$aMesserschmidt, J.$$b1
000038404 7001_ $$0P:(DE-HGF)0$$avon Bohlen, A.$$b2
000038404 7001_ $$0P:(DE-Juel1)129343$$aKastenholz, B.$$b3$$uFZJ
000038404 7001_ $$0P:(DE-Juel1)129325$$aGünther, K.$$b4$$uFZJ
000038404 773__ $$0PERI:(DE-600)1475486-1$$a10.1002/elps.200305833$$gVol. 25, p. 1758 - 1764$$p1758 - 1764$$q25<1758 - 1764$$tElectrophoresis$$v25$$x0173-0835$$y2004
000038404 909CO $$ooai:juser.fz-juelich.de:38404$$pVDB
000038404 9131_ $$0G:(DE-Juel1)FUEK257$$bEnvironment (Umwelt)$$kU01$$lChemie und Dynamik der Geo-Biosphäre$$vChemie und Dynamik der Geo-Biosphäre$$x0
000038404 9141_ $$y2004
000038404 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000038404 9201_ $$0I:(DE-Juel1)VDB49$$d31.12.2006$$gICG$$kICG-III$$lPhytosphäre$$x0
000038404 970__ $$aVDB:(DE-Juel1)49153
000038404 980__ $$aVDB
000038404 980__ $$aConvertedRecord
000038404 980__ $$ajournal
000038404 980__ $$aI:(DE-Juel1)IBG-2-20101118
000038404 980__ $$aUNRESTRICTED
000038404 981__ $$aI:(DE-Juel1)IBG-2-20101118
000038404 981__ $$aI:(DE-Juel1)ICG-3-20090406