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@ARTICLE{Weber:38404,
      author       = {Weber, G. and Messerschmidt, J. and von Bohlen, A. and
                      Kastenholz, B. and Günther, K.},
      title        = {{I}mproved separation of palladium species in biological
                      matrices by using a combination of gel permeation
                      chromatography and isotachophoresis},
      journal      = {Electrophoresis},
      volume       = {25},
      issn         = {0173-0835},
      address      = {Weinheim},
      publisher    = {Wiley-Blackwell},
      reportid     = {PreJuSER-38404},
      pages        = {1758 - 1764},
      year         = {2004},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {The binding of palladium to high-molecular-mass compounds
                      in palladium-treated lettuce is investigated as an example
                      for a biological matrix. The total palladium concentration
                      in lettuce leaves is 10.3 ng/g wet weight. After
                      homogenization, high-molecular-mass compounds (> 10 kDa) are
                      isolated by ultrafiltration. For separation of these
                      palladium species a combination of preparative gel
                      permeation chromatography (GPC) and preparative
                      isotachophoresis (ITP) is used. Palladium is determined in
                      separated fractions by using a highly sensitive total
                      reflection X-ray fluorescence (TXRF) method after
                      preconcentration. After GPC separation, four main fractions
                      of palladium species are collected, each containing
                      palladium in ng quantities (3-10 ng). Two of these fractions
                      are further separated by ITP, yielding at least three main
                      peaks per GPC fraction, each containing palladium in the
                      range of 0.3-3 ng. These palladium containing peaks are
                      characterized by high-performance size exclusion
                      chromatography (HPSEC) and capillary isotachophoresis (cITP)
                      in parallel. HPSEC enables the estimation of the molecular
                      mass of six main palladium peaks, covering a molecular mass
                      range of 69-200 kDa. It is also shown that the estimation of
                      molecular mass after separation is more reliable than the
                      respective estimation directly in the first GPC run.
                      However, cITP reveals that each of the separated peaks is
                      still a mixture of at least five different compounds.},
      keywords     = {Chromatography, Gel / Electrophoresis / Fluorescence /
                      Lettuce: chemistry / Palladium: chemistry / Plant Leaves:
                      chemistry / Ultrafiltration / X-Rays / Palladium (NLM
                      Chemicals) / J (WoSType)},
      cin          = {ICG-III},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB49},
      pnm          = {Chemie und Dynamik der Geo-Biosphäre},
      pid          = {G:(DE-Juel1)FUEK257},
      shelfmark    = {Biochemical Research Methods / Chemistry, Analytical},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:15213973},
      UT           = {WOS:000222396000005},
      doi          = {10.1002/elps.200305833},
      url          = {https://juser.fz-juelich.de/record/38404},
}