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000038569 0247_ $$2pmid$$apmid:15268620
000038569 0247_ $$2DOI$$a10.1063/1.1644799
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000038569 084__ $$2WoS$$aPhysics, Atomic, Molecular & Chemical
000038569 1001_ $$0P:(DE-Juel1)130797$$aLettinga, M. P.$$b0$$uFZJ
000038569 245__ $$aRotational dynamics of colloidal spheresprobed with fluorescence recovery after photobleaching
000038569 260__ $$aMelville, NY$$bAmerican Institute of Physics$$c2004
000038569 300__ $$a4517 - 4529
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000038569 440_0 $$03145$$aJournal of Chemical Physics$$v120$$x0021-9606$$y9
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000038569 520__ $$aWe report a polarized fluorescence recovery after photobleaching (pFRAP) method to measure the rotational dynamics of fluorescent colloids over a wide dynamic range. The method is based on the polarization anisotropy in the fluorescence intensity, generated by bleaching of fluorescently labeled particles with an intense pulse of linearly polarized laser light. The rotational mobilities of the fluorescent particles can be extracted from the relaxation kinetics of the postbleach fluorescence polarization anisotropy. Our pFRAP setup has access to correlation times over a range of time scales from tens of microseconds to tens of seconds, and is highly sensitive, so very low concentrations of labeled particles can be probed. We present a detailed description of the theoretical background of pFRAP. The performance of the equipment is demonstrated for fluorescent colloidal silica spheres, dispersed in pure solvents as well as in fd-virus suspensions.
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000038569 650_2 $$2MeSH$$aBiomechanics
000038569 650_2 $$2MeSH$$aColloids: analysis
000038569 650_2 $$2MeSH$$aColloids: chemistry
000038569 650_2 $$2MeSH$$aComputer Simulation
000038569 650_2 $$2MeSH$$aDiffusion
000038569 650_2 $$2MeSH$$aFluorescence Recovery After Photobleaching: methods
000038569 650_2 $$2MeSH$$aMicrospheres
000038569 650_2 $$2MeSH$$aModels, Chemical
000038569 650_2 $$2MeSH$$aModels, Molecular
000038569 650_2 $$2MeSH$$aMolecular Conformation
000038569 650_2 $$2MeSH$$aRotation
000038569 650_2 $$2MeSH$$aStress, Mechanical
000038569 650_7 $$00$$2NLM Chemicals$$aColloids
000038569 650_7 $$2WoSType$$aJ
000038569 7001_ $$0P:(DE-HGF)0$$aKoenderink, G. H.$$b1
000038569 7001_ $$0P:(DE-HGF)0$$aKuipers, B. W. M.$$b2
000038569 7001_ $$0P:(DE-HGF)0$$aBessels, E.$$b3
000038569 7001_ $$0P:(DE-HGF)0$$aPhilipse, R. H.$$b4
000038569 773__ $$0PERI:(DE-600)1473050-9$$a10.1063/1.1644799$$gVol. 120, p. 4517 - 4529$$p4517 - 4529$$q120<4517 - 4529$$tThe @journal of chemical physics$$v120$$x0021-9606$$y2004
000038569 8567_ $$uhttp://hdl.handle.net/2128/1186$$uhttp://dx.doi.org/10.1063/1.1644799
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