TY  - JOUR
AU  - Lettinga, M. P.
AU  - Koenderink, G. H.
AU  - Kuipers, B. W. M.
AU  - Bessels, E.
AU  - Philipse, R. H.
TI  - Rotational dynamics of colloidal spheresprobed with fluorescence recovery after photobleaching
JO  - The journal of chemical physics
VL  - 120
SN  - 0021-9606
CY  - Melville, NY
PB  - American Institute of Physics
M1  - PreJuSER-38569
SP  - 4517 - 4529
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - We report a polarized fluorescence recovery after photobleaching (pFRAP) method to measure the rotational dynamics of fluorescent colloids over a wide dynamic range. The method is based on the polarization anisotropy in the fluorescence intensity, generated by bleaching of fluorescently labeled particles with an intense pulse of linearly polarized laser light. The rotational mobilities of the fluorescent particles can be extracted from the relaxation kinetics of the postbleach fluorescence polarization anisotropy. Our pFRAP setup has access to correlation times over a range of time scales from tens of microseconds to tens of seconds, and is highly sensitive, so very low concentrations of labeled particles can be probed. We present a detailed description of the theoretical background of pFRAP. The performance of the equipment is demonstrated for fluorescent colloidal silica spheres, dispersed in pure solvents as well as in fd-virus suspensions.
KW  - Biomechanics
KW  - Colloids: analysis
KW  - Colloids: chemistry
KW  - Computer Simulation
KW  - Diffusion
KW  - Fluorescence Recovery After Photobleaching: methods
KW  - Microspheres
KW  - Models, Chemical
KW  - Models, Molecular
KW  - Molecular Conformation
KW  - Rotation
KW  - Stress, Mechanical
KW  - Colloids (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15268620
UR  - <Go to ISI:>//WOS:000189209200050
DO  - DOI:10.1063/1.1644799
UR  - https://juser.fz-juelich.de/record/38569
ER  -