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@ARTICLE{Lettinga:38569,
      author       = {Lettinga, M. P. and Koenderink, G. H. and Kuipers, B. W. M.
                      and Bessels, E. and Philipse, R. H.},
      title        = {{R}otational dynamics of colloidal spheresprobed with
                      fluorescence recovery after photobleaching},
      journal      = {The journal of chemical physics},
      volume       = {120},
      issn         = {0021-9606},
      address      = {Melville, NY},
      publisher    = {American Institute of Physics},
      reportid     = {PreJuSER-38569},
      pages        = {4517 - 4529},
      year         = {2004},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {We report a polarized fluorescence recovery after
                      photobleaching (pFRAP) method to measure the rotational
                      dynamics of fluorescent colloids over a wide dynamic range.
                      The method is based on the polarization anisotropy in the
                      fluorescence intensity, generated by bleaching of
                      fluorescently labeled particles with an intense pulse of
                      linearly polarized laser light. The rotational mobilities of
                      the fluorescent particles can be extracted from the
                      relaxation kinetics of the postbleach fluorescence
                      polarization anisotropy. Our pFRAP setup has access to
                      correlation times over a range of time scales from tens of
                      microseconds to tens of seconds, and is highly sensitive, so
                      very low concentrations of labeled particles can be probed.
                      We present a detailed description of the theoretical
                      background of pFRAP. The performance of the equipment is
                      demonstrated for fluorescent colloidal silica spheres,
                      dispersed in pure solvents as well as in fd-virus
                      suspensions.},
      keywords     = {Biomechanics / Colloids: analysis / Colloids: chemistry /
                      Computer Simulation / Diffusion / Fluorescence Recovery
                      After Photobleaching: methods / Microspheres / Models,
                      Chemical / Models, Molecular / Molecular Conformation /
                      Rotation / Stress, Mechanical / Colloids (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IFF-IWM},
      ddc          = {540},
      cid          = {I:(DE-Juel1)VDB343},
      pnm          = {Kondensierte Materie},
      pid          = {G:(DE-Juel1)FUEK242},
      shelfmark    = {Physics, Atomic, Molecular $\&$ Chemical},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:15268620},
      UT           = {WOS:000189209200050},
      doi          = {10.1063/1.1644799},
      url          = {https://juser.fz-juelich.de/record/38569},
}