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| 001 | 38569 | ||
| 005 | 20240619083405.0 | ||
| 017 | _ | _ | |a This version is available at the following Publisher URL: http://jcp.aip.org |
| 024 | 7 | _ | |a pmid:15268620 |2 pmid |
| 024 | 7 | _ | |a 10.1063/1.1644799 |2 DOI |
| 024 | 7 | _ | |a WOS:000189209200050 |2 WOS |
| 024 | 7 | _ | |a 2128/1186 |2 Handle |
| 037 | _ | _ | |a PreJuSER-38569 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 540 |
| 084 | _ | _ | |2 WoS |a Physics, Atomic, Molecular & Chemical |
| 100 | 1 | _ | |a Lettinga, M. P. |b 0 |u FZJ |0 P:(DE-Juel1)130797 |
| 245 | _ | _ | |a Rotational dynamics of colloidal spheresprobed with fluorescence recovery after photobleaching |
| 260 | _ | _ | |a Melville, NY |b American Institute of Physics |c 2004 |
| 300 | _ | _ | |a 4517 - 4529 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Journal of Chemical Physics |x 0021-9606 |0 3145 |y 9 |v 120 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a We report a polarized fluorescence recovery after photobleaching (pFRAP) method to measure the rotational dynamics of fluorescent colloids over a wide dynamic range. The method is based on the polarization anisotropy in the fluorescence intensity, generated by bleaching of fluorescently labeled particles with an intense pulse of linearly polarized laser light. The rotational mobilities of the fluorescent particles can be extracted from the relaxation kinetics of the postbleach fluorescence polarization anisotropy. Our pFRAP setup has access to correlation times over a range of time scales from tens of microseconds to tens of seconds, and is highly sensitive, so very low concentrations of labeled particles can be probed. We present a detailed description of the theoretical background of pFRAP. The performance of the equipment is demonstrated for fluorescent colloidal silica spheres, dispersed in pure solvents as well as in fd-virus suspensions. |
| 536 | _ | _ | |a Kondensierte Materie |c M02 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK242 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Biomechanics |
| 650 | _ | 2 | |2 MeSH |a Colloids: analysis |
| 650 | _ | 2 | |2 MeSH |a Colloids: chemistry |
| 650 | _ | 2 | |2 MeSH |a Computer Simulation |
| 650 | _ | 2 | |2 MeSH |a Diffusion |
| 650 | _ | 2 | |2 MeSH |a Fluorescence Recovery After Photobleaching: methods |
| 650 | _ | 2 | |2 MeSH |a Microspheres |
| 650 | _ | 2 | |2 MeSH |a Models, Chemical |
| 650 | _ | 2 | |2 MeSH |a Models, Molecular |
| 650 | _ | 2 | |2 MeSH |a Molecular Conformation |
| 650 | _ | 2 | |2 MeSH |a Rotation |
| 650 | _ | 2 | |2 MeSH |a Stress, Mechanical |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Colloids |
| 650 | _ | 7 | |a J |2 WoSType |
| 700 | 1 | _ | |a Koenderink, G. H. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Kuipers, B. W. M. |b 2 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Bessels, E. |b 3 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Philipse, R. H. |b 4 |0 P:(DE-HGF)0 |
| 773 | _ | _ | |a 10.1063/1.1644799 |g Vol. 120, p. 4517 - 4529 |p 4517 - 4529 |q 120<4517 - 4529 |0 PERI:(DE-600)1473050-9 |t The @journal of chemical physics |v 120 |y 2004 |x 0021-9606 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1063/1.1644799 |u http://hdl.handle.net/2128/1186 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/38569/files/49769.pdf |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/38569/files/49769.jpg?subformat=icon-1440 |x icon-1440 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/38569/files/49769.jpg?subformat=icon-180 |x icon-180 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/38569/files/49769.jpg?subformat=icon-640 |x icon-640 |y OpenAccess |
| 909 | C | O | |o oai:juser.fz-juelich.de:38569 |p openaire |p open_access |p driver |p VDB |p dnbdelivery |
| 913 | 1 | _ | |k M02 |v Kondensierte Materie |l Kondensierte Materie |b Materie |0 G:(DE-Juel1)FUEK242 |x 0 |
| 914 | 1 | _ | |y 2004 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 915 | _ | _ | |2 StatID |0 StatID:(DE-HGF)0510 |a OpenAccess |
| 920 | 1 | _ | |k IFF-IWM |l Weiche Materie |d 31.12.2006 |g IFF |0 I:(DE-Juel1)VDB343 |x 0 |
| 970 | _ | _ | |a VDB:(DE-Juel1)49769 |
| 980 | 1 | _ | |a FullTexts |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a JUWEL |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-3-20110106 |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a FullTexts |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-3-20110106 |
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