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000003955 0247_ $$2pmid$$apmid:19388624
000003955 0247_ $$2DOI$$a10.1021/la8041023
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000003955 041__ $$aeng
000003955 082__ $$a670
000003955 084__ $$2WoS$$aChemistry, Multidisciplinary
000003955 084__ $$2WoS$$aChemistry, Physical
000003955 084__ $$2WoS$$aMaterials Science, Multidisciplinary
000003955 1001_ $$0P:(DE-Juel1)128805$$aCsiszar, A.$$b0$$uFZJ
000003955 245__ $$aDouble shell giant vesicles mimicking Gram-negative cell wall behaviour during dehydration
000003955 260__ $$aWashington, DC$$bACS Publ.$$c2009
000003955 300__ $$a5753 - 5761
000003955 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000003955 440_0 $$04081$$aLangmuir$$v25$$x0743-7463$$y10
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000003955 520__ $$aA biomimetic system modeling the behavior of Gram-negative bacteria under hyperosmotic stress was developed. To this end, we introduced a two-step electroswelling procedure for encapsulation of giant unilamellar vesicles by an additional membrane. Both membranes of the resulting double-shell vesicles (DSVs) were fluid. Additionally, the outer membrane was rigidified by a monolayer of streptavidin forming a two-dimensional crystal. For strong attachment of this protein layer, the outer membrane contained biotinylated lipids. This reinforced protein-lipid compound membrane served to model the assembly of the murein wall and outer membrane of Gram-negative bacteria. We characterized DSVs by confocal laser scanning microscopy. Furthermore, DSVs were exposed to hyperosmotic media (osmotic difference 0-1100 mosm/L), and the resulting shapes were analyzed. DSVs coated with streptavidin were much less deformed or destroyed by osmotic stress than bare DSVs or DSVs coated with noncrystalline avidin. Osmotically stressed DSVs coated with streptavidin displayed weak wrinkling of the outer membrane and formed small daughter vesicles of the inner membrane. Both features and the toughness against hyperosmotic stress are well described characteristics of Gram-negative bacteria.
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000003955 588__ $$aDataset connected to Web of Science, Pubmed
000003955 650_2 $$2MeSH$$aBiomimetic Materials: chemistry
000003955 650_2 $$2MeSH$$aCell Membrane
000003955 650_2 $$2MeSH$$aCell Wall
000003955 650_2 $$2MeSH$$aGram-Negative Bacteria
000003955 650_2 $$2MeSH$$aOsmotic Pressure
000003955 650_2 $$2MeSH$$aPeptidoglycan: chemistry
000003955 650_2 $$2MeSH$$aStreptavidin: chemistry
000003955 650_7 $$00$$2NLM Chemicals$$aPeptidoglycan
000003955 650_7 $$09013-20-1$$2NLM Chemicals$$aStreptavidin
000003955 650_7 $$2WoSType$$aJ
000003955 7001_ $$0P:(DE-Juel1)VDB27696$$aHoffmann, B.$$b1$$uFZJ
000003955 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b2$$uFZJ
000003955 773__ $$0PERI:(DE-600)2005937-1$$a10.1021/la8041023$$gVol. 25, p. 5753 - 5761$$p5753 - 5761$$q25<5753 - 5761$$tLangmuir$$v25$$x0743-7463$$y2009
000003955 8567_ $$uhttp://dx.doi.org/10.1021/la8041023
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000003955 9131_ $$0G:(DE-Juel1)FUEK414$$bMaterie$$kP54$$lKondensierte Materie$$vKondensierte Materie$$x0$$zentfällt   bis 2009
000003955 9141_ $$y2009
000003955 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000003955 9201_ $$0I:(DE-Juel1)VDB802$$d31.12.2010$$gIBN$$kIBN-4$$lBiomechanik$$x0
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