TY  - JOUR
AU  - Csiszar, A.
AU  - Hoffmann, B.
AU  - Merkel, R.
TI  - Double shell giant vesicles mimicking Gram-negative cell wall behaviour during dehydration
JO  - Langmuir
VL  - 25
SN  - 0743-7463
CY  - Washington, DC
PB  - ACS Publ.
M1  - PreJuSER-3955
SP  - 5753 - 5761
PY  - 2009
N1  - Record converted from VDB: 12.11.2012
AB  - A biomimetic system modeling the behavior of Gram-negative bacteria under hyperosmotic stress was developed. To this end, we introduced a two-step electroswelling procedure for encapsulation of giant unilamellar vesicles by an additional membrane. Both membranes of the resulting double-shell vesicles (DSVs) were fluid. Additionally, the outer membrane was rigidified by a monolayer of streptavidin forming a two-dimensional crystal. For strong attachment of this protein layer, the outer membrane contained biotinylated lipids. This reinforced protein-lipid compound membrane served to model the assembly of the murein wall and outer membrane of Gram-negative bacteria. We characterized DSVs by confocal laser scanning microscopy. Furthermore, DSVs were exposed to hyperosmotic media (osmotic difference 0-1100 mosm/L), and the resulting shapes were analyzed. DSVs coated with streptavidin were much less deformed or destroyed by osmotic stress than bare DSVs or DSVs coated with noncrystalline avidin. Osmotically stressed DSVs coated with streptavidin displayed weak wrinkling of the outer membrane and formed small daughter vesicles of the inner membrane. Both features and the toughness against hyperosmotic stress are well described characteristics of Gram-negative bacteria.
KW  - Biomimetic Materials: chemistry
KW  - Cell Membrane
KW  - Cell Wall
KW  - Gram-Negative Bacteria
KW  - Osmotic Pressure
KW  - Peptidoglycan: chemistry
KW  - Streptavidin: chemistry
KW  - Peptidoglycan (NLM Chemicals)
KW  - Streptavidin (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19388624
UR  - <Go to ISI:>//WOS:000266081000048
DO  - DOI:10.1021/la8041023
UR  - https://juser.fz-juelich.de/record/3955
ER  -