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@ARTICLE{Csiszar:3955,
      author       = {Csiszar, A. and Hoffmann, B. and Merkel, R.},
      title        = {{D}ouble shell giant vesicles mimicking {G}ram-negative
                      cell wall behaviour during dehydration},
      journal      = {Langmuir},
      volume       = {25},
      issn         = {0743-7463},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {PreJuSER-3955},
      pages        = {5753 - 5761},
      year         = {2009},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {A biomimetic system modeling the behavior of Gram-negative
                      bacteria under hyperosmotic stress was developed. To this
                      end, we introduced a two-step electroswelling procedure for
                      encapsulation of giant unilamellar vesicles by an additional
                      membrane. Both membranes of the resulting double-shell
                      vesicles (DSVs) were fluid. Additionally, the outer membrane
                      was rigidified by a monolayer of streptavidin forming a
                      two-dimensional crystal. For strong attachment of this
                      protein layer, the outer membrane contained biotinylated
                      lipids. This reinforced protein-lipid compound membrane
                      served to model the assembly of the murein wall and outer
                      membrane of Gram-negative bacteria. We characterized DSVs by
                      confocal laser scanning microscopy. Furthermore, DSVs were
                      exposed to hyperosmotic media (osmotic difference 0-1100
                      mosm/L), and the resulting shapes were analyzed. DSVs coated
                      with streptavidin were much less deformed or destroyed by
                      osmotic stress than bare DSVs or DSVs coated with
                      noncrystalline avidin. Osmotically stressed DSVs coated with
                      streptavidin displayed weak wrinkling of the outer membrane
                      and formed small daughter vesicles of the inner membrane.
                      Both features and the toughness against hyperosmotic stress
                      are well described characteristics of Gram-negative
                      bacteria.},
      keywords     = {Biomimetic Materials: chemistry / Cell Membrane / Cell Wall
                      / Gram-Negative Bacteria / Osmotic Pressure / Peptidoglycan:
                      chemistry / Streptavidin: chemistry / Peptidoglycan (NLM
                      Chemicals) / Streptavidin (NLM Chemicals) / J (WoSType)},
      cin          = {IBN-4},
      ddc          = {670},
      cid          = {I:(DE-Juel1)VDB802},
      pnm          = {Kondensierte Materie},
      pid          = {G:(DE-Juel1)FUEK414},
      shelfmark    = {Chemistry, Multidisciplinary / Chemistry, Physical /
                      Materials Science, Multidisciplinary},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:19388624},
      UT           = {WOS:000266081000048},
      doi          = {10.1021/la8041023},
      url          = {https://juser.fz-juelich.de/record/3955},
}