Home > Publications database > Double shell giant vesicles mimicking Gram-negative cell wall behaviour during dehydration > print

001     3955
005     20200402205555.0
024 7 _ |2 pmid
|a pmid:19388624
024 7 _ |2 DOI
|a 10.1021/la8041023
024 7 _ |2 WOS
|a WOS:000266081000048
037 _ _ |a PreJuSER-3955
041 _ _ |a eng
082 _ _ |a 670
084 _ _ |2 WoS
|a Chemistry, Multidisciplinary
084 _ _ |2 WoS
|a Chemistry, Physical
084 _ _ |2 WoS
|a Materials Science, Multidisciplinary
100 1 _ |a Csiszar, A.
|b 0
|u FZJ
|0 P:(DE-Juel1)128805
245 _ _ |a Double shell giant vesicles mimicking Gram-negative cell wall behaviour during dehydration
260 _ _ |a Washington, DC
|b ACS Publ.
|c 2009
300 _ _ |a 5753 - 5761
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Langmuir
|x 0743-7463
|0 4081
|y 10
|v 25
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a A biomimetic system modeling the behavior of Gram-negative bacteria under hyperosmotic stress was developed. To this end, we introduced a two-step electroswelling procedure for encapsulation of giant unilamellar vesicles by an additional membrane. Both membranes of the resulting double-shell vesicles (DSVs) were fluid. Additionally, the outer membrane was rigidified by a monolayer of streptavidin forming a two-dimensional crystal. For strong attachment of this protein layer, the outer membrane contained biotinylated lipids. This reinforced protein-lipid compound membrane served to model the assembly of the murein wall and outer membrane of Gram-negative bacteria. We characterized DSVs by confocal laser scanning microscopy. Furthermore, DSVs were exposed to hyperosmotic media (osmotic difference 0-1100 mosm/L), and the resulting shapes were analyzed. DSVs coated with streptavidin were much less deformed or destroyed by osmotic stress than bare DSVs or DSVs coated with noncrystalline avidin. Osmotically stressed DSVs coated with streptavidin displayed weak wrinkling of the outer membrane and formed small daughter vesicles of the inner membrane. Both features and the toughness against hyperosmotic stress are well described characteristics of Gram-negative bacteria.
536 _ _ |a Kondensierte Materie
|c P54
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK414
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Biomimetic Materials: chemistry
650 _ 2 |2 MeSH
|a Cell Membrane
650 _ 2 |2 MeSH
|a Cell Wall
650 _ 2 |2 MeSH
|a Gram-Negative Bacteria
650 _ 2 |2 MeSH
|a Osmotic Pressure
650 _ 2 |2 MeSH
|a Peptidoglycan: chemistry
650 _ 2 |2 MeSH
|a Streptavidin: chemistry
650 _ 7 |0 0
|2 NLM Chemicals
|a Peptidoglycan
650 _ 7 |0 9013-20-1
|2 NLM Chemicals
|a Streptavidin
650 _ 7 |a J
|2 WoSType
700 1 _ |a Hoffmann, B.
|b 1
|u FZJ
|0 P:(DE-Juel1)VDB27696
700 1 _ |a Merkel, R.
|b 2
|u FZJ
|0 P:(DE-Juel1)128833
773 _ _ |a 10.1021/la8041023
|g Vol. 25, p. 5753 - 5761
|p 5753 - 5761
|q 25<5753 - 5761
|0 PERI:(DE-600)2005937-1
|t Langmuir
|v 25
|y 2009
|x 0743-7463
856 7 _ |u http://dx.doi.org/10.1021/la8041023
909 C O |o oai:juser.fz-juelich.de:3955
|p VDB
913 1 _ |k P54
|v Kondensierte Materie
|l Kondensierte Materie
|b Materie
|z entfällt bis 2009
|0 G:(DE-Juel1)FUEK414
|x 0
914 1 _ |y 2009
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k IBN-4
|l Biomechanik
|d 31.12.2010
|g IBN
|0 I:(DE-Juel1)VDB802
|x 0
970 _ _ |a VDB:(DE-Juel1)110517
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)ICS-7-20110106
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBI-2-20200312
981 _ _ |a I:(DE-Juel1)ICS-7-20110106

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