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@ARTICLE{Klauth:40216,
      author       = {Klauth, P. and Wilhelm, R. and Klumpp, E. and Poschen, L.
                      and Groeneweg, J.},
      title        = {{E}numeration of soil bacteria with the green fluorescent
                      nucleic acid dye {S}ytox green in the presence of soil
                      particles},
      journal      = {Journal of microbiological methods},
      volume       = {59},
      issn         = {0167-7012},
      address      = {New York, NY},
      publisher    = {Elsevier},
      reportid     = {PreJuSER-40216},
      pages        = {189 - 198},
      year         = {2004},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Total counts in soils are usually determined using
                      fluorescent dyes, such as DAPI or Sybr green, due to
                      fluorescence enhancement if they are bound to nucleic acids.
                      Unfortunately, these commonly used dyes stain soil particles
                      as well. Therefore, besides fluorescence enhancement,
                      sufficient spectral differentiation is also required. We
                      present a new procedure that overcomes the problems of
                      visualising bacteria on surfaces in soil and avoids the
                      separation of soil particles to a large extent. Spectral
                      differentiation between bacteria and soil matrix is achieved
                      by using Sytox green and a suboptimal excitation wavelength.
                      Bacteria exhibit a bright green fluorescence, while soil
                      particles fluoresce blue or red. Slight homogenisation and
                      sedimentation of the sand and coarse silt that were too big
                      for microscopic investigations were the only separation
                      steps required. We compared the proposed Sytox green
                      staining with Sybr green staining. The recovery of Sybr
                      green-stained cells amounted to $38\%,$ whereas in samples
                      stained by Sytox green $81\%$ of the spiked cells were
                      counted. Sytox green can also be combined with fluorescence
                      in situ hybridisation (FISH) using deep red dyes such as
                      Cy5.},
      keywords     = {Aluminum Silicates / Bacillus subtilis: isolation $\&$
                      purification / Bacillus subtilis: metabolism / Cell
                      Membrane: metabolism / Fluorescent Dyes: chemistry /
                      Fluorescent Dyes: metabolism / Microscopy, Fluorescence:
                      methods / Organic Chemicals / Pseudomonas: isolation $\&$
                      purification / Pseudomonas: metabolism / Ralstonia:
                      isolation $\&$ purification / Ralstonia: metabolism / Soil
                      Microbiology / Spectrophotometry, Ultraviolet / Aluminum
                      Silicates (NLM Chemicals) / Fluorescent Dyes (NLM Chemicals)
                      / Organic Chemicals (NLM Chemicals) / SYTOX Green (NLM
                      Chemicals) / clay (NLM Chemicals) / J (WoSType)},
      cin          = {ICG-IV},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB50},
      pnm          = {Chemie und Dynamik der Geo-Biosphäre},
      pid          = {G:(DE-Juel1)FUEK257},
      shelfmark    = {Biochemical Research Methods / Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:15369855},
      UT           = {WOS:000224363000005},
      doi          = {10.1016/j.mimet.2004.07.004},
      url          = {https://juser.fz-juelich.de/record/40216},
}