TY  - JOUR
AU  - Efremov, R.
AU  - Moukhametzianov, R.
AU  - Büldt, G.
AU  - Gordeliy, V. I.
TI  - Physical Detwinning of Hemihedrally Twinned Hexagonal Crystals of Bacteriorhdopsin
JO  - Biophysical journal
VL  - 87
SN  - 0006-3495
CY  - New York, NY
PB  - Rockefeller Univ. Press
M1  - PreJuSER-40230
SP  - 3608 - 3613
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - Hexagonal crystals of the membrane protein bacteriorhodopsin of space group P6 3 grown in lipidic cubic phase are twinned hemihedrally. It was shown that slow changes of salt concentration in the mother liquor lead to a split of crystals so that the split parts preserved high diffraction quality. Analysis of diffraction data from split crystals by Yeates statistic and Britton plot showed that the split parts are free of twinning. It is concluded that crystals of bacteriorhodopsin are composed of several macroscopic twinning domains with sizes comparable to the original crystal. The appearance of twinning domains during crystal growth and the mechanism of splitting are discussed.
KW  - Bacteriorhodopsins: analysis
KW  - Bacteriorhodopsins: chemistry
KW  - Bacteriorhodopsins: ultrastructure
KW  - Binding Sites
KW  - Computer Simulation
KW  - Crystallization: methods
KW  - Crystallography: methods
KW  - Models, Chemical
KW  - Models, Molecular
KW  - Multiprotein Complexes: chemistry
KW  - Multiprotein Complexes: ultrastructure
KW  - Protein Binding
KW  - Multiprotein Complexes (NLM Chemicals)
KW  - Bacteriorhodopsins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15339801
C2  - pmc:PMC1304826
UR  - <Go to ISI:>//WOS:000224732500061
DO  - DOI:10.1529/biophysj.104.046573
UR  - https://juser.fz-juelich.de/record/40230
ER  -