000040468 001__ 40468
000040468 005__ 20180210133050.0
000040468 0247_ $$2DOI$$a10.1007/s00374-003-0680-8
000040468 0247_ $$2WOS$$aWOS:000187186400010
000040468 037__ $$aPreJuSER-40468
000040468 041__ $$aeng
000040468 082__ $$a630
000040468 084__ $$2WoS$$aSoil Science
000040468 1001_ $$0P:(DE-HGF)0$$aRupela, O. P.$$b0
000040468 245__ $$aA novel method for the identification and enumeration of microorganisms with potential for suppressing fungal plant pathogens
000040468 260__ $$aBerlin$$bSpringer$$c2003
000040468 300__ $$a131 - 134
000040468 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000040468 3367_ $$2DataCite$$aOutput Types/Journal article
000040468 3367_ $$00$$2EndNote$$aJournal Article
000040468 3367_ $$2BibTeX$$aARTICLE
000040468 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000040468 3367_ $$2DRIVER$$aarticle
000040468 440_0 $$0856$$aBiology and Fertility of Soils$$v39$$x0178-2762$$y2
000040468 500__ $$aRecord converted from VDB: 12.11.2012
000040468 520__ $$aThis paper describes a method that allowed counting of both the total culturable and antagonistic microorganisms in a given source such as compost. Fusarium solani, used as the test fungus, was spread-plated on quarter-strength (1/4) potato dextrose agar (PDA), its surface was exposed in a laminar flow for 4 h and then another layer (2-3 mm thick) of 1/4 PDA was poured over it, on which an appropriate dilution of a compost sample was spread-plated. Microorganisms in the compost samples appeared first, and were counted as total culturable organisms. Plates were further incubated until F. solani grew through the upper layer of PDA (generally in 4-8 days) and covered the whole plate including most of the microbial colonies, except for a few which had a halo around them. These were counted as antagonistic, and they were isolated and purified for further studies. The population of bacteria in the six specific compost samples (called Biodynamic or BD preparations by organic farmers) ranged from 3.45 log(10) (in BD502) to 8.59 log(10) (in BD504) per gram of materials. The population of antagonistic bacteria was counted for three of the six compost samples, and ranged from 3.24 log(10) (in BD502) to 6.90 log(10) (in BD500). Of the 67 bacterial isolates showing a halo that were assembled from different sources, 17 suppressed at least 1 of the 4 plant pathogenic fungi against which these were evaluated using the dual culture method.
000040468 536__ $$0G:(DE-Juel1)FUEK257$$2G:(DE-HGF)$$aChemie und Dynamik der Geo-Biosphäre$$cU01$$x0
000040468 588__ $$aDataset connected to Web of Science
000040468 650_7 $$2WoSType$$aJ
000040468 65320 $$2Author$$atwo-layer method
000040468 65320 $$2Author$$adual plate method
000040468 65320 $$2Author$$aenumeration
000040468 65320 $$2Author$$aantagonistic microorganisms
000040468 65320 $$2Author$$aFusarium solani
000040468 65320 $$2Author$$abiodynamic preparations
000040468 7001_ $$0P:(DE-HGF)0$$aGopalakrishnan, S.$$b1
000040468 7001_ $$0P:(DE-Juel1)VDB4034$$aKrajewski, M.$$b2$$uFZJ
000040468 7001_ $$0P:(DE-HGF)0$$aSriveni, M.$$b3
000040468 773__ $$0PERI:(DE-600)1473419-9$$a10.1007/s00374-003-0680-8$$gVol. 39, p. 131 - 134$$p131 - 134$$q39<131 - 134$$tBiology and fertility of soils$$v39$$x0178-2762$$y2003
000040468 8567_ $$uhttp://dx.doi.org/10.1007/s00374-003-0680-8
000040468 909CO $$ooai:juser.fz-juelich.de:40468$$pVDB
000040468 9131_ $$0G:(DE-Juel1)FUEK257$$bEnvironment (Umwelt)$$kU01$$lChemie und Dynamik der Geo-Biosphäre$$vChemie und Dynamik der Geo-Biosphäre$$x0
000040468 9141_ $$aNachtrag$$y2003
000040468 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000040468 9201_ $$0I:(DE-Juel1)VDB50$$d31.12.2006$$gICG$$kICG-IV$$lAgrosphäre$$x0
000040468 970__ $$aVDB:(DE-Juel1)54390
000040468 980__ $$aVDB
000040468 980__ $$aConvertedRecord
000040468 980__ $$ajournal
000040468 980__ $$aI:(DE-Juel1)IBG-3-20101118
000040468 980__ $$aUNRESTRICTED
000040468 981__ $$aI:(DE-Juel1)IBG-3-20101118