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@ARTICLE{Rupela:40468,
      author       = {Rupela, O. P. and Gopalakrishnan, S. and Krajewski, M. and
                      Sriveni, M.},
      title        = {{A} novel method for the identification and enumeration of
                      microorganisms with potential for suppressing fungal plant
                      pathogens},
      journal      = {Biology and fertility of soils},
      volume       = {39},
      issn         = {0178-2762},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {PreJuSER-40468},
      pages        = {131 - 134},
      year         = {2003},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {This paper describes a method that allowed counting of both
                      the total culturable and antagonistic microorganisms in a
                      given source such as compost. Fusarium solani, used as the
                      test fungus, was spread-plated on quarter-strength (1/4)
                      potato dextrose agar (PDA), its surface was exposed in a
                      laminar flow for 4 h and then another layer (2-3 mm thick)
                      of 1/4 PDA was poured over it, on which an appropriate
                      dilution of a compost sample was spread-plated.
                      Microorganisms in the compost samples appeared first, and
                      were counted as total culturable organisms. Plates were
                      further incubated until F. solani grew through the upper
                      layer of PDA (generally in 4-8 days) and covered the whole
                      plate including most of the microbial colonies, except for a
                      few which had a halo around them. These were counted as
                      antagonistic, and they were isolated and purified for
                      further studies. The population of bacteria in the six
                      specific compost samples (called Biodynamic or BD
                      preparations by organic farmers) ranged from 3.45 log(10)
                      (in BD502) to 8.59 log(10) (in BD504) per gram of materials.
                      The population of antagonistic bacteria was counted for
                      three of the six compost samples, and ranged from 3.24
                      log(10) (in BD502) to 6.90 log(10) (in BD500). Of the 67
                      bacterial isolates showing a halo that were assembled from
                      different sources, 17 suppressed at least 1 of the 4 plant
                      pathogenic fungi against which these were evaluated using
                      the dual culture method.},
      keywords     = {J (WoSType)},
      cin          = {ICG-IV},
      ddc          = {630},
      cid          = {I:(DE-Juel1)VDB50},
      pnm          = {Chemie und Dynamik der Geo-Biosphäre},
      pid          = {G:(DE-Juel1)FUEK257},
      shelfmark    = {Soil Science},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000187186400010},
      doi          = {10.1007/s00374-003-0680-8},
      url          = {https://juser.fz-juelich.de/record/40468},
}