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001     40468
005     20180210133050.0
024 7 _ |2 DOI
|a 10.1007/s00374-003-0680-8
024 7 _ |2 WOS
|a WOS:000187186400010
037 _ _ |a PreJuSER-40468
041 _ _ |a eng
082 _ _ |a 630
084 _ _ |2 WoS
|a Soil Science
100 1 _ |a Rupela, O. P.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a A novel method for the identification and enumeration of microorganisms with potential for suppressing fungal plant pathogens
260 _ _ |a Berlin
|b Springer
|c 2003
300 _ _ |a 131 - 134
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Biology and Fertility of Soils
|x 0178-2762
|0 856
|y 2
|v 39
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a This paper describes a method that allowed counting of both the total culturable and antagonistic microorganisms in a given source such as compost. Fusarium solani, used as the test fungus, was spread-plated on quarter-strength (1/4) potato dextrose agar (PDA), its surface was exposed in a laminar flow for 4 h and then another layer (2-3 mm thick) of 1/4 PDA was poured over it, on which an appropriate dilution of a compost sample was spread-plated. Microorganisms in the compost samples appeared first, and were counted as total culturable organisms. Plates were further incubated until F. solani grew through the upper layer of PDA (generally in 4-8 days) and covered the whole plate including most of the microbial colonies, except for a few which had a halo around them. These were counted as antagonistic, and they were isolated and purified for further studies. The population of bacteria in the six specific compost samples (called Biodynamic or BD preparations by organic farmers) ranged from 3.45 log(10) (in BD502) to 8.59 log(10) (in BD504) per gram of materials. The population of antagonistic bacteria was counted for three of the six compost samples, and ranged from 3.24 log(10) (in BD502) to 6.90 log(10) (in BD500). Of the 67 bacterial isolates showing a halo that were assembled from different sources, 17 suppressed at least 1 of the 4 plant pathogenic fungi against which these were evaluated using the dual culture method.
536 _ _ |a Chemie und Dynamik der Geo-Biosphäre
|c U01
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK257
|x 0
588 _ _ |a Dataset connected to Web of Science
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a two-layer method
653 2 0 |2 Author
|a dual plate method
653 2 0 |2 Author
|a enumeration
653 2 0 |2 Author
|a antagonistic microorganisms
653 2 0 |2 Author
|a Fusarium solani
653 2 0 |2 Author
|a biodynamic preparations
700 1 _ |a Gopalakrishnan, S.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Krajewski, M.
|b 2
|u FZJ
|0 P:(DE-Juel1)VDB4034
700 1 _ |a Sriveni, M.
|b 3
|0 P:(DE-HGF)0
773 _ _ |a 10.1007/s00374-003-0680-8
|g Vol. 39, p. 131 - 134
|p 131 - 134
|q 39<131 - 134
|0 PERI:(DE-600)1473419-9
|t Biology and fertility of soils
|v 39
|y 2003
|x 0178-2762
856 7 _ |u http://dx.doi.org/10.1007/s00374-003-0680-8
909 C O |o oai:juser.fz-juelich.de:40468
|p VDB
913 1 _ |k U01
|v Chemie und Dynamik der Geo-Biosphäre
|l Chemie und Dynamik der Geo-Biosphäre
|b Environment (Umwelt)
|0 G:(DE-Juel1)FUEK257
|x 0
914 1 _ |a Nachtrag
|y 2003
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k ICG-IV
|l Agrosphäre
|d 31.12.2006
|g ICG
|0 I:(DE-Juel1)VDB50
|x 0
970 _ _ |a VDB:(DE-Juel1)54390
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)IBG-3-20101118
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBG-3-20101118

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