TY  - JOUR
AU  - Matsubara, S.
AU  - Chow, W. S.
TI  - Populations of photoinactivated photosystem II reaction ceners characterized by chlorophyll a fluorescence lifetime in vivo
JO  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 101
SN  - 0027-8424
CY  - Washington, DC
PB  - Academy
M1  - PreJuSER-41136
SP  - 18234 - 18239
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - Photosystem (PS) II centers, which split water into oxygen, protons, and electrons during photosynthesis, require light but are paradoxically inactivated by it. Prolonged light exposure concomitantly decreased both the functional fraction of PSII reaction centers and the integral PSII chlorophyll (Chl) a fluorescence lifetime in leaf segments of Capsicum annuum L. Acceleration of photoinactivation of PSII by a pretreatment with the inhibitors/uncoupler lincomycin, DTT, or nigericin further reduced PSII Chl a fluorescence lifetimes. A global analysis of fluorescence lifetime distributions revealed the presence of at least two distinct populations of photoinactivated PSII centers, one at 1.25 ns, and the other at 0.58 ns. Light treatment first increased the 1.25-ns component, a weak quencher, at the expense of a component at 2.22 ns corresponding to functional PSII centers. The 0.58-ns component, a strong quencher, emerged later than the 1.25-ns component. The strongly quenching PSII reaction centers could serve to avoid further damage to themselves and protect their functional neighbors by acting as strong energy sinks.
KW  - Anti-Bacterial Agents: pharmacology
KW  - Capsicum: metabolism
KW  - Chlorophyll: chemistry
KW  - Dithiothreitol: pharmacology
KW  - Light
KW  - Light-Harvesting Protein Complexes: chemistry
KW  - Lincomycin: pharmacology
KW  - Microscopy, Fluorescence: methods
KW  - Models, Biological
KW  - Nigericin: pharmacology
KW  - Oxygen: metabolism
KW  - Photons
KW  - Photosynthesis
KW  - Photosynthetic Reaction Center Complex Proteins: chemistry
KW  - Photosystem II Protein Complex: chemistry
KW  - Protons
KW  - Temperature
KW  - Time Factors
KW  - Xanthophylls: metabolism
KW  - Anti-Bacterial Agents (NLM Chemicals)
KW  - Light-Harvesting Protein Complexes (NLM Chemicals)
KW  - Photosynthetic Reaction Center Complex Proteins (NLM Chemicals)
KW  - Photosystem II Protein Complex (NLM Chemicals)
KW  - Protons (NLM Chemicals)
KW  - Xanthophylls (NLM Chemicals)
KW  - Chlorophyll (NLM Chemicals)
KW  - Lincomycin (NLM Chemicals)
KW  - Nigericin (NLM Chemicals)
KW  - Dithiothreitol (NLM Chemicals)
KW  - chlorophyll a (NLM Chemicals)
KW  - Oxygen (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15601775
C2  - pmc:PMC539729
UR  - <Go to ISI:>//WOS:000226102700063
DO  - DOI:10.1073/pnas.0403857102
UR  - https://juser.fz-juelich.de/record/41136
ER  -