TY  - JOUR
AU  - Ataka, K.
AU  - Heberle, J.
TI  - Functional vibrational spectroscopy of a cytochrome c monolayer: SEIDAS probes the interaction with different surface modified electrodes
JO  - Journal of the American Chemical Society
VL  - 126
SN  - 0002-7863
CY  - Washington, DC
PB  - American Chemical Society
M1  - PreJuSER-41691
SP  - 9445 - 9457
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - Electrochemically induced infrared difference spectra of cytochrome c on various chemically modified electrodes (CMEs) are recorded by exploiting the surface-enhancement exerted by a granular gold film. We have recently developed surface-enhanced infrared difference absorption spectroscopy (SEIDAS), which provides acute sensitivity to observe the minute enzymatic change of a protein on the level of a monolayer. By these means, we demonstrate that the relative band intensities in the potential-induced difference spectra of adsorbed cytochrome c are significantly dependent on the type of CME used (mercaptopropionic acid, mercaptoethanol, 4,4'-dithiodipyridine, or L-cysteine). These differences are attributed to the altered interaction of cytochrome c with the headgroup of the various CMEs leading to variations in surface orientation and relative distance from the surface. Nevertheless, the peak positions of the observed bands are identical among the CMEs employed. This implies that the internal conformational changes induced by the redox reaction of the adsorbed cytochrome c are not disturbed by the interaction with the CME and that full functionality of the protein is retained. Finally, we critically discuss our results within the framework of the different models for cytochrome c adsorption on CMEs.
KW  - Crystallography, X-Ray
KW  - Cytochromes c: chemistry
KW  - Electrochemistry: instrumentation
KW  - Electrochemistry: methods
KW  - Electrodes
KW  - Gold: chemistry
KW  - Nuclear Magnetic Resonance, Biomolecular
KW  - Oxidation-Reduction
KW  - Spectroscopy, Fourier Transform Infrared: instrumentation
KW  - Spectroscopy, Fourier Transform Infrared: methods
KW  - Gold (NLM Chemicals)
KW  - Cytochromes c (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15281838
UR  - <Go to ISI:>//WOS:000222950900057
DO  - DOI:10.1021/ja048346n
UR  - https://juser.fz-juelich.de/record/41691
ER  -