TY  - JOUR
AU  - Meyburg, S.
AU  - Goryll, M.
AU  - Moers, J.
AU  - Ingebrandt, S.
AU  - Böcker-Meffert, S.
AU  - Lüth, H.
AU  - Offenhäusser, A.
TI  - N-Channel Field Effect Transistors with Floating Gates for Extracellular Recordings
JO  - Biosensors and bioelectronics
VL  - 21
SN  - 0956-5663
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-42877
SP  - 1037 - 1044
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - A field-effect transistor (FET) for recording extracellular signals from electrogenic cells is presented. The so-called floating gate architecture combines a complementary metal oxide semiconductor (CMOS)-type n-channel transistor with an independent sensing area. This concept allows the transistor and sensing area to be optimised separately. The devices are robust and can be reused several times. The noise level of the devices was smaller than of comparable non-metallised gate FETs. In addition to the usual drift of FET devices, we observed a long-term drift that has to be controlled for future long-term measurements. The device performance for extracellular signal recording was tested using embryonic rat cardiac myocytes cultured on fibronectin-coated chips. The extracellular cell signals were recorded before and after the addition of the cardioactive isoproterenol. The signal shapes of the measured action potentials were comparable to the non-metallised gate FETs previously used in similar experiments. The fabrication of the devices involved the process steps of standard CMOS that were necessary to create n-channel transistors. The implementation of a complete CMOS process would facilitate the integration of the logical circuits necessary for signal pre-processing on a chip, which is a prerequisite for a greater number of sensor spots in future layouts.
KW  - Action Potentials: physiology
KW  - Amplifiers, Electronic
KW  - Animals
KW  - Biosensing Techniques: instrumentation
KW  - Cells, Cultured
KW  - Equipment Design
KW  - Equipment Failure Analysis
KW  - Membrane Potentials: physiology
KW  - Microelectrodes
KW  - Myocytes, Cardiac: physiology
KW  - Rats
KW  - Rats, Wistar
KW  - Reproducibility of Results
KW  - Sensitivity and Specificity
KW  - Transistors, Electronic
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16029948
UR  - <Go to ISI:>//WOS:000234644400007
DO  - DOI:10.1016/j.bios.2005.03.010
UR  - https://juser.fz-juelich.de/record/42877
ER  -