TY  - JOUR
AU  - Ataka, K.
AU  - Giess, F.
AU  - Knoll, W.
AU  - Naumann, R.
AU  - Haber-Pohlmeier, S.
AU  - Richter, B.
AU  - Heberle, J.
TI  - Oriented attachment and membrane reconstitution of His-tagged cytochrome c oxidase to a gold electrode: in-situ monitoring by Surface Enhanced Infrared Absorption Spectroscopy
JO  - Journal of the American Chemical Society
VL  - 126
SN  - 0002-7863
CY  - Washington, DC
PB  - American Chemical Society
M1  - PreJuSER-43304
SP  - 16199 - 16206
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - A novel concept is introduced for the oriented incorporation of membrane proteins into solid supported lipid bilayers. Recombinant cytochrome c oxidase solubilized in detergent was immobilized on a chemically modified gold surface via the affinity of its histidine-tag to a nickel-chelating nitrilo-triacetic acid (NTA) surface. The oriented protein monolayer was reconstituted into the lipid environment by detergent substitution. The individual steps of the surface modification, including (1) chemical modification of the gold support, (2) adsorption of the protein, and (3) reconstitution of the lipid bilayer, were followed in situ by means of surface-enhanced infrared absorption spectroscopy (SEIRAS) and accompanied by normal-mode analysis. The high surface sensitivity of SEIRAS allows for the identification of each chemical reaction process within the monolayer at the molecular level. Finally, full functionality of the surface-tethered cytochrome c oxidase was demonstrated by cyclic voltammetry after binding of the natural electron donor cytochrome c.
KW  - Electrochemistry
KW  - Electron Transport Complex IV: chemistry
KW  - Enzymes, Immobilized: chemistry
KW  - Gold: chemistry
KW  - Histidine: chemistry
KW  - Lipid Bilayers: chemistry
KW  - Models, Molecular
KW  - Nitrilotriacetic Acid: chemistry
KW  - Recombinant Proteins: chemistry
KW  - Spectroscopy, Fourier Transform Infrared: methods
KW  - Surface Properties
KW  - Enzymes, Immobilized (NLM Chemicals)
KW  - Lipid Bilayers (NLM Chemicals)
KW  - Recombinant Proteins (NLM Chemicals)
KW  - Nitrilotriacetic Acid (NLM Chemicals)
KW  - Histidine (NLM Chemicals)
KW  - Gold (NLM Chemicals)
KW  - Electron Transport Complex IV (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15584756
UR  - <Go to ISI:>//WOS:000225697000055
DO  - DOI:10.1021/ja045951h
UR  - https://juser.fz-juelich.de/record/43304
ER  -