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@ARTICLE{Merkel:44598,
      author       = {Merkel, R. and Endress, E. and Bayerl, S. and Prechtel, K.
                      and Maier, C. and Bayerl, T. M.},
      title        = {{T}he effect of cholesterol, lanosterol and ergosterol on
                      lecithin bilayer mechanical properties at molecular and
                      microscopic dimensions : a solid-state {NMR} and micropipet
                      study},
      journal      = {Langmuir},
      volume       = {18},
      issn         = {0743-7463},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {PreJuSER-44598},
      pages        = {3293 - 3299},
      year         = {2002},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Lecithin (DPPC) membranes doped with high concentrations
                      (40 $mol\%)$ of three biologically relevant sterols
                      (cholesterol, lanosterol, and ergosterol) were studied by
                      spectroscopic and force measurements. Micropipet aspiration
                      measurements of giant unilamellar vesicles provided the
                      membrane area expansion modulus K on the microscopic scale
                      while deuterium NMR experiments gave parameters such as the
                      line shape, transverse relaxation, and molecular order,
                      which are all based on a molecular scale at a given time
                      resolution, Micropipet experiments at 10 degreesC gave K
                      values for the three samples with ergosterol > cholesterol >
                      lanosterol. From the NMR we obtained the highest
                      CH2-segmental molecular order and longest transverse
                      relaxation time for cholesterol and the opposite for
                      ergosterol. The lanosterol NMR parameters were found to be
                      between those of the other two sterols but were closer to
                      those of cholesterol. The extent of deformation of the
                      sterol-doped vesicles in the magnetic field of the NMR
                      magnet was lanosterol > cholesterol > ergosterol. The
                      results suggest that membrane micromechanic properties are
                      manifest in solid-state NMR parameters and suggest that high
                      K values at the microscopic scale correspond to high
                      molecular order and long transverse relaxation times at the
                      molecular scale. Furthermore, the extent of magnetic field
                      orientation of vesicles measured by NMR correlated well with
                      K determined by micropipet aspiration. The results may help
                      to appreciate why cholesterol has gained a dominant role
                      over the other sterols in the course of cellular evolution.},
      keywords     = {J (WoSType)},
      cin          = {ISG-4},
      ddc          = {670},
      cid          = {I:(DE-Juel1)VDB44},
      pnm          = {Kondensierte Materie},
      pid          = {G:(DE-Juel1)FUEK242},
      shelfmark    = {Chemistry, Multidisciplinary / Chemistry, Physical /
                      Materials Science, Multidisciplinary},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000174978000053},
      doi          = {10.1021/la011596m},
      url          = {https://juser.fz-juelich.de/record/44598},
}