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@ARTICLE{Vanderborght:452,
author = {Vanderborght, J. and Gahwiller, P. and Wydler, H. and
Schultze, U. and Flühler, M. O.},
title = {{I}maging fluorescent dye concentrations on soil surfaces :
uncertainty of concentration estimates},
journal = {Soil Science Society of America journal},
volume = {66},
issn = {0361-5995},
address = {Madison, Wis.},
publisher = {SSSA},
reportid = {PreJuSER-452},
pages = {760 - 773},
year = {2002},
note = {Record converted from VDB: 12.11.2012},
abstract = {To investigate transport processes in soils, detailed
information about the spatial distribution of solutes is
required. We describe a method to obtain concentration maps
of fluorescent tracers on cross sections of soil cores with
a high spatial resolution. The fluorescence signal of two
dye tracers, brilliant sulfaflavine (BF;
1-H-Benz(de)isoquinoline-5-sulfonic acid,
2,3-dihydro-6-amino-1,3-dioxo-2-(p-tolyl)-, monosodium salt)
and sulforhodamine B (SB; xanthylium,
3,6-bis(diethylamino)-9-(2,4-disulfophenyl)-, inner salt,
sodium salt), was imaged on the exposed cross sections. The
fluorescence signal was corrected for variable illumination
light intensity and optical properties of the soil across
the exposed surface. Correction factors for varying optical
soil properties were derived from the image of the reflected
excitation light at the exposed surface. Linear calibration
relations related the corrected fluorescence image to the
total tracer concentration (C-t) map, that is, mass of dye
dissolved in the soil solution and sorbed to the soil
particles per unit volume bulk soil. Corrections for varying
optical properties of the soil surface were important to
reduce the uncertainty of the concentration that was
estimated from the fluorescence signal. For BF, the
calibration relations were different for different soil
materials and a soil specific calibration had to be used.
Variations in background fluorescence were an important
source of uncertainty of the BF concentration estimates but
can be overcome by applying higher concentrations. For SB,
variations in calibration relations and in the background
fluorescence were considerably smaller, and so is the
uncertainty of the estimated SB concentrations.},
keywords = {J (WoSType)},
cin = {ICG-IV},
ddc = {550},
cid = {I:(DE-Juel1)VDB50},
pnm = {Chemie und Dynamik der Geo-Biosphäre},
pid = {G:(DE-Juel1)FUEK257},
shelfmark = {Soil Science},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000175288300011},
url = {https://juser.fz-juelich.de/record/452},
}