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| 001 | 45390 | ||
| 005 | 20200402205930.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:15817164 |
| 024 | 7 | _ | |2 DOI |a 10.1016/j.yexcr.2005.01.012 |
| 024 | 7 | _ | |2 WOS |a WOS:000228409100016 |
| 037 | _ | _ | |a PreJuSER-45390 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 084 | _ | _ | |2 WoS |a Oncology |
| 084 | _ | _ | |2 WoS |a Cell Biology |
| 100 | 1 | _ | |a Vetterkind, S. |b 0 |0 P:(DE-HGF)0 |
| 245 | _ | _ | |a Binding of Par-4 to the actin cytoskeleton is essential for Par-4/Dlk-mediated apoptosis |
| 260 | _ | _ | |a Orlando, Fla. |b Academic Press |c 2005 |
| 300 | _ | _ | |a 392 - 408 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Experimental Cell Research |x 0014-4827 |0 13781 |v 305 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a Prostate apoptosis response-4 (Par-4) is a 38-kDa protein originally identified as a gene product upregulated in prostate cancer cells undergoing apoptosis. Cell death mediated by Par-4 and its interaction partner DAP like kinase (Dlk) is characterized by dramatic changes of the cytoskeleton. To uncover the role of the cytoskeleton in Par-4/Dlk-mediated apoptosis, we analyzed Par-4 for a direct association with cytoskeletal structures. Confocal fluorescence microscopy revealed that endogenous Par-4 is specifically associated with stress fibers in rat fibroblasts. In vitro cosedimentation analyses and in vivo FRET analyses showed that Par-4 directly binds to F-actin. Actin binding is mediated by the N-terminal 266 amino acids, but does not require the C-terminal region of Par-4 containing the leucine zipper and the death domain. Furthermore, the interaction of Par-4 with actin filaments leads to the formation of actin bundles in vitro and in vivo. In rat fibroblasts, this microfilament association is essential for the pro-apoptotic function of Par-4, since both disruption of the actin cytoskeleton by cytochalasin D treatment and overexpression of Par-4 constructs impaired in actin binding result in a significant decrease of apoptosis induction by Par-4 and Dlk. We propose a model, in which Par-4 recruits Dlk to stress fibers, leading to enhanced phosphorylation of the regulatory light chain of myosin II (MLC) and to the induction of apoptosis. |
| 536 | _ | _ | |a Neurowissenschaften |c L01 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK255 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Actin Cytoskeleton: chemistry |
| 650 | _ | 2 | |2 MeSH |a Actin Cytoskeleton: metabolism |
| 650 | _ | 2 | |2 MeSH |a Actins: analysis |
| 650 | _ | 2 | |2 MeSH |a Actins: metabolism |
| 650 | _ | 2 | |2 MeSH |a Animals |
| 650 | _ | 2 | |2 MeSH |a Apoptosis: physiology |
| 650 | _ | 2 | |2 MeSH |a Apoptosis Regulatory Proteins |
| 650 | _ | 2 | |2 MeSH |a Calcium-Calmodulin-Dependent Protein Kinases |
| 650 | _ | 2 | |2 MeSH |a Cardiac Myosins: metabolism |
| 650 | _ | 2 | |2 MeSH |a Cell Line, Tumor |
| 650 | _ | 2 | |2 MeSH |a Humans |
| 650 | _ | 2 | |2 MeSH |a Intracellular Signaling Peptides and Proteins: analysis |
| 650 | _ | 2 | |2 MeSH |a Intracellular Signaling Peptides and Proteins: genetics |
| 650 | _ | 2 | |2 MeSH |a Intracellular Signaling Peptides and Proteins: metabolism |
| 650 | _ | 2 | |2 MeSH |a MAP Kinase Kinase Kinases |
| 650 | _ | 2 | |2 MeSH |a Male |
| 650 | _ | 2 | |2 MeSH |a Mice |
| 650 | _ | 2 | |2 MeSH |a Mutation: genetics |
| 650 | _ | 2 | |2 MeSH |a Myosin Light Chains: metabolism |
| 650 | _ | 2 | |2 MeSH |a Phosphorylation |
| 650 | _ | 2 | |2 MeSH |a Protein-Serine-Threonine Kinases: metabolism |
| 650 | _ | 2 | |2 MeSH |a Rats |
| 650 | _ | 2 | |2 MeSH |a Up-Regulation |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Actins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Apoptosis Regulatory Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Intracellular Signaling Peptides and Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Myosin Light Chains |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a myosin light chain 2 |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a prostate apoptosis response-4 protein |
| 650 | _ | 7 | |0 EC 2.7.11.1 |2 NLM Chemicals |a Protein-Serine-Threonine Kinases |
| 650 | _ | 7 | |0 EC 2.7.11.1 |2 NLM Chemicals |a death-associated protein kinase |
| 650 | _ | 7 | |0 EC 2.7.11.17 |2 NLM Chemicals |a Calcium-Calmodulin-Dependent Protein Kinases |
| 650 | _ | 7 | |0 EC 2.7.11.25 |2 NLM Chemicals |a MAP Kinase Kinase Kinases |
| 650 | _ | 7 | |0 EC 2.7.11.25 |2 NLM Chemicals |a mitogen-activated protein kinase kinase kinase 12 |
| 650 | _ | 7 | |0 EC 3.6.1.- |2 NLM Chemicals |a Cardiac Myosins |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a actin |
| 653 | 2 | 0 | |2 Author |a cytoskeleton |
| 653 | 2 | 0 | |2 Author |a Par-4 |
| 653 | 2 | 0 | |2 Author |a Dlk |
| 653 | 2 | 0 | |2 Author |a apoptosis |
| 700 | 1 | _ | |a Illenberger, S. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Kubicek, J. |b 2 |u FZJ |0 P:(DE-Juel1)VDB10481 |
| 700 | 1 | _ | |a Boosen, M. |b 3 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Appel, S. |b 4 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Naim, H. Y. |b 5 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Scheidtmann, K.-H. |b 6 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Preuss, U. |b 7 |0 P:(DE-HGF)0 |
| 773 | _ | _ | |a 10.1016/j.yexcr.2005.01.012 |g Vol. 305, p. 392 - 408 |p 392 - 408 |q 305<392 - 408 |0 PERI:(DE-600)1466780-0 |t Experimental cell research |v 305 |y 2005 |x 0014-4827 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1016/j.yexcr.2005.01.012 |
| 909 | C | O | |o oai:juser.fz-juelich.de:45390 |p VDB |
| 913 | 1 | _ | |k L01 |v Neurowissenschaften |l Funktion und Dysfunktion des Nervensystems |b Leben |0 G:(DE-Juel1)FUEK255 |x 0 |
| 914 | 1 | _ | |y 2005 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k IBI-2 |l Biologische Strukturforschung |d 31.12.2006 |g IBI |0 I:(DE-Juel1)VDB58 |x 0 |
| 970 | _ | _ | |a VDB:(DE-Juel1)68947 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-7-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
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