000045521 001__ 45521
000045521 005__ 20200423204117.0
000045521 0247_ $$2pmid$$apmid:16269752
000045521 0247_ $$2pmc$$apmc:PMC1287687
000045521 0247_ $$2DOI$$a10.1128/AEM.71.11.7139-7144.2005
000045521 0247_ $$2WOS$$aWOS:000233225000083
000045521 0247_ $$2Handle$$a2128/2423
000045521 0247_ $$2altmetric$$aaltmetric:21813686
000045521 037__ $$aPreJuSER-45521
000045521 041__ $$aeng
000045521 082__ $$a570
000045521 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000045521 084__ $$2WoS$$aMicrobiology
000045521 1001_ $$0P:(DE-Juel1)VDB1238$$aPeters-Wendisch, P.$$b0$$uFZJ
000045521 245__ $$aMetabolic Engineering of Corynebacterium glutamicum for L-Serine production
000045521 260__ $$aWashington, DC [u.a.]$$bSoc.$$c2005
000045521 300__ $$a7139 - 7144
000045521 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000045521 3367_ $$2DataCite$$aOutput Types/Journal article
000045521 3367_ $$00$$2EndNote$$aJournal Article
000045521 3367_ $$2BibTeX$$aARTICLE
000045521 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000045521 3367_ $$2DRIVER$$aarticle
000045521 440_0 $$08561$$aApplied and Environmental Microbiology$$v71$$x0099-2240$$y11
000045521 500__ $$aRecord converted from VDB: 12.11.2012
000045521 520__ $$aAlthough L-serine proceeds in just three steps from the glycolytic intermediate 3-phosphoglycerate, and as much as 8% of the carbon assimilated from glucose is directed via L-serine formation, previous attempts to obtain a strain producing L-serine from glucose have not been successful. We functionally identified the genes serC and serB from Corynebacterium glutamicum, coding for phosphoserine aminotransferase and phosphoserine phosphatase, respectively. The overexpression of these genes, together with the third biosynthetic serA gene, serA(delta197), encoding an L-serine-insensitive 3-phosphoglycerate dehydrogenase, yielded only traces of L-serine, as did the overexpression of these genes in a strain with the L-serine dehydratase gene sdaA deleted. However, reduced expression of the serine hydroxymethyltransferase gene glyA, in combination with the overexpression of serA(delta197), serC, and serB, resulted in a transient accumulation of up to 16 mM L-serine in the culture medium. When sdaA was also deleted, the resulting strain, C. glutamicum delta sdaA::pK18mobglyA'(pEC-T18mob2serA(delta197)CB), accumulated up to 86 mM L-serine with a maximal specific productivity of 1.2 mmol h(-1) g (dry weight)(-1). This illustrates a high rate of L-serine formation and also utilization in the C. glutamicum wild type. Therefore, metabolic engineering of L-serine production from glucose can be achieved only by addressing the apparent key position of this amino acid in the central metabolism.
000045521 536__ $$0G:(DE-Juel1)FUEK256$$2G:(DE-HGF)$$aBiotechnologie$$cL02$$x0
000045521 588__ $$aDataset connected to Web of Science, Pubmed
000045521 650_2 $$2MeSH$$aCorynebacterium glutamicum: enzymology
000045521 650_2 $$2MeSH$$aCorynebacterium glutamicum: genetics
000045521 650_2 $$2MeSH$$aCulture Media
000045521 650_2 $$2MeSH$$aGene Deletion
000045521 650_2 $$2MeSH$$aGene Expression Regulation, Bacterial
000045521 650_2 $$2MeSH$$aGenetic Engineering: methods
000045521 650_2 $$2MeSH$$aGlycine Hydroxymethyltransferase: genetics
000045521 650_2 $$2MeSH$$aGlycine Hydroxymethyltransferase: metabolism
000045521 650_2 $$2MeSH$$aL-Serine Dehydratase: genetics
000045521 650_2 $$2MeSH$$aL-Serine Dehydratase: metabolism
000045521 650_2 $$2MeSH$$aPhosphoglycerate Dehydrogenase: genetics
000045521 650_2 $$2MeSH$$aPhosphoglycerate Dehydrogenase: metabolism
000045521 650_2 $$2MeSH$$aPhosphoric Monoester Hydrolases: genetics
000045521 650_2 $$2MeSH$$aPhosphoric Monoester Hydrolases: metabolism
000045521 650_2 $$2MeSH$$aSerine: biosynthesis
000045521 650_2 $$2MeSH$$aTransaminases: genetics
000045521 650_2 $$2MeSH$$aTransaminases: metabolism
000045521 650_7 $$00$$2NLM Chemicals$$aCulture Media
000045521 650_7 $$056-45-1$$2NLM Chemicals$$aSerine
000045521 650_7 $$0EC 1.1.1.95$$2NLM Chemicals$$aPhosphoglycerate Dehydrogenase
000045521 650_7 $$0EC 2.1.2.1$$2NLM Chemicals$$aGlycine Hydroxymethyltransferase
000045521 650_7 $$0EC 2.6.1.-$$2NLM Chemicals$$aTransaminases
000045521 650_7 $$0EC 2.6.1.52$$2NLM Chemicals$$aphosphoserine aminotransferase
000045521 650_7 $$0EC 3.1.3.-$$2NLM Chemicals$$aPhosphoric Monoester Hydrolases
000045521 650_7 $$0EC 3.1.3.3$$2NLM Chemicals$$aphosphoserine phosphatase
000045521 650_7 $$0EC 4.3.1.17$$2NLM Chemicals$$aL-Serine Dehydratase
000045521 650_7 $$2WoSType$$aJ
000045521 7001_ $$0P:(DE-Juel1)VDB28996$$aStolz, M.$$b1$$uFZJ
000045521 7001_ $$0P:(DE-Juel1)VDB25622$$aEtterich, H.$$b2$$uFZJ
000045521 7001_ $$0P:(DE-Juel1)VDB2921$$aKennerknecht, N.$$b3$$uFZJ
000045521 7001_ $$0P:(DE-Juel1)128985$$aSahm, H.$$b4$$uFZJ
000045521 7001_ $$0P:(DE-Juel1)VDB57928$$aEggeling, L.$$b5$$uFZJ
000045521 773__ $$0PERI:(DE-600)1478346-0$$a10.1128/AEM.71.11.7139-7144.2005$$gVol. 71, p. 7139 - 7144$$p7139 - 7144$$q71<7139 - 7144$$tApplied and environmental microbiology$$v71$$x0099-2240$$y2005
000045521 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1287687
000045521 8564_ $$uhttps://juser.fz-juelich.de/record/45521/files/69626.pdf$$yOpenAccess
000045521 8564_ $$uhttps://juser.fz-juelich.de/record/45521/files/69626.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000045521 8564_ $$uhttps://juser.fz-juelich.de/record/45521/files/69626.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000045521 8564_ $$uhttps://juser.fz-juelich.de/record/45521/files/69626.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000045521 909CO $$ooai:juser.fz-juelich.de:45521$$pdnbdelivery$$pVDB$$pdriver$$popen_access$$popenaire
000045521 9131_ $$0G:(DE-Juel1)FUEK256$$bLeben$$kL02$$lBiotechnologie$$vBiotechnologie$$x0
000045521 9141_ $$y2005
000045521 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000045521 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000045521 9201_ $$0I:(DE-Juel1)VDB55$$gIBT$$kIBT-1$$lBiotechnologie 1$$x0$$zab 31.10.10 weitergeführt als IBG-1
000045521 970__ $$aVDB:(DE-Juel1)69626
000045521 980__ $$aVDB
000045521 980__ $$aJUWEL
000045521 980__ $$aConvertedRecord
000045521 980__ $$ajournal
000045521 980__ $$aI:(DE-Juel1)IBG-1-20101118
000045521 980__ $$aUNRESTRICTED
000045521 980__ $$aFullTexts
000045521 9801_ $$aFullTexts
000045521 981__ $$aI:(DE-Juel1)IBG-1-20101118