TY  - JOUR
AU  - Peters-Wendisch, P.
AU  - Stolz, M.
AU  - Etterich, H.
AU  - Kennerknecht, N.
AU  - Sahm, H.
AU  - Eggeling, L.
TI  - Metabolic Engineering of Corynebacterium glutamicum for L-Serine production
JO  - Applied and environmental microbiology
VL  - 71
SN  - 0099-2240
CY  - Washington, DC [u.a.]
PB  - Soc.
M1  - PreJuSER-45521
SP  - 7139 - 7144
PY  - 2005
N1  - Record converted from VDB: 12.11.2012
AB  - Although L-serine proceeds in just three steps from the glycolytic intermediate 3-phosphoglycerate, and as much as 8% of the carbon assimilated from glucose is directed via L-serine formation, previous attempts to obtain a strain producing L-serine from glucose have not been successful. We functionally identified the genes serC and serB from Corynebacterium glutamicum, coding for phosphoserine aminotransferase and phosphoserine phosphatase, respectively. The overexpression of these genes, together with the third biosynthetic serA gene, serA(delta197), encoding an L-serine-insensitive 3-phosphoglycerate dehydrogenase, yielded only traces of L-serine, as did the overexpression of these genes in a strain with the L-serine dehydratase gene sdaA deleted. However, reduced expression of the serine hydroxymethyltransferase gene glyA, in combination with the overexpression of serA(delta197), serC, and serB, resulted in a transient accumulation of up to 16 mM L-serine in the culture medium. When sdaA was also deleted, the resulting strain, C. glutamicum delta sdaA::pK18mobglyA'(pEC-T18mob2serA(delta197)CB), accumulated up to 86 mM L-serine with a maximal specific productivity of 1.2 mmol h(-1) g (dry weight)(-1). This illustrates a high rate of L-serine formation and also utilization in the C. glutamicum wild type. Therefore, metabolic engineering of L-serine production from glucose can be achieved only by addressing the apparent key position of this amino acid in the central metabolism.
KW  - Corynebacterium glutamicum: enzymology
KW  - Corynebacterium glutamicum: genetics
KW  - Culture Media
KW  - Gene Deletion
KW  - Gene Expression Regulation, Bacterial
KW  - Genetic Engineering: methods
KW  - Glycine Hydroxymethyltransferase: genetics
KW  - Glycine Hydroxymethyltransferase: metabolism
KW  - L-Serine Dehydratase: genetics
KW  - L-Serine Dehydratase: metabolism
KW  - Phosphoglycerate Dehydrogenase: genetics
KW  - Phosphoglycerate Dehydrogenase: metabolism
KW  - Phosphoric Monoester Hydrolases: genetics
KW  - Phosphoric Monoester Hydrolases: metabolism
KW  - Serine: biosynthesis
KW  - Transaminases: genetics
KW  - Transaminases: metabolism
KW  - Culture Media (NLM Chemicals)
KW  - Serine (NLM Chemicals)
KW  - Phosphoglycerate Dehydrogenase (NLM Chemicals)
KW  - Glycine Hydroxymethyltransferase (NLM Chemicals)
KW  - Transaminases (NLM Chemicals)
KW  - phosphoserine aminotransferase (NLM Chemicals)
KW  - Phosphoric Monoester Hydrolases (NLM Chemicals)
KW  - phosphoserine phosphatase (NLM Chemicals)
KW  - L-Serine Dehydratase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16269752
C2  - pmc:PMC1287687
UR  - <Go to ISI:>//WOS:000233225000083
DO  - DOI:10.1128/AEM.71.11.7139-7144.2005
UR  - https://juser.fz-juelich.de/record/45521
ER  -