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@ARTICLE{PetersWendisch:45521,
      author       = {Peters-Wendisch, P. and Stolz, M. and Etterich, H. and
                      Kennerknecht, N. and Sahm, H. and Eggeling, L.},
      title        = {{M}etabolic {E}ngineering of {C}orynebacterium glutamicum
                      for {L}-{S}erine production},
      journal      = {Applied and environmental microbiology},
      volume       = {71},
      issn         = {0099-2240},
      address      = {Washington, DC [u.a.]},
      publisher    = {Soc.},
      reportid     = {PreJuSER-45521},
      pages        = {7139 - 7144},
      year         = {2005},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Although L-serine proceeds in just three steps from the
                      glycolytic intermediate 3-phosphoglycerate, and as much as
                      $8\%$ of the carbon assimilated from glucose is directed via
                      L-serine formation, previous attempts to obtain a strain
                      producing L-serine from glucose have not been successful. We
                      functionally identified the genes serC and serB from
                      Corynebacterium glutamicum, coding for phosphoserine
                      aminotransferase and phosphoserine phosphatase,
                      respectively. The overexpression of these genes, together
                      with the third biosynthetic serA gene, serA(delta197),
                      encoding an L-serine-insensitive 3-phosphoglycerate
                      dehydrogenase, yielded only traces of L-serine, as did the
                      overexpression of these genes in a strain with the L-serine
                      dehydratase gene sdaA deleted. However, reduced expression
                      of the serine hydroxymethyltransferase gene glyA, in
                      combination with the overexpression of serA(delta197), serC,
                      and serB, resulted in a transient accumulation of up to 16
                      mM L-serine in the culture medium. When sdaA was also
                      deleted, the resulting strain, C. glutamicum delta
                      sdaA::pK18mobglyA'(pEC-T18mob2serA(delta197)CB), accumulated
                      up to 86 mM L-serine with a maximal specific productivity of
                      1.2 mmol h(-1) g (dry weight)(-1). This illustrates a high
                      rate of L-serine formation and also utilization in the C.
                      glutamicum wild type. Therefore, metabolic engineering of
                      L-serine production from glucose can be achieved only by
                      addressing the apparent key position of this amino acid in
                      the central metabolism.},
      keywords     = {Corynebacterium glutamicum: enzymology / Corynebacterium
                      glutamicum: genetics / Culture Media / Gene Deletion / Gene
                      Expression Regulation, Bacterial / Genetic Engineering:
                      methods / Glycine Hydroxymethyltransferase: genetics /
                      Glycine Hydroxymethyltransferase: metabolism / L-Serine
                      Dehydratase: genetics / L-Serine Dehydratase: metabolism /
                      Phosphoglycerate Dehydrogenase: genetics / Phosphoglycerate
                      Dehydrogenase: metabolism / Phosphoric Monoester Hydrolases:
                      genetics / Phosphoric Monoester Hydrolases: metabolism /
                      Serine: biosynthesis / Transaminases: genetics /
                      Transaminases: metabolism / Culture Media (NLM Chemicals) /
                      Serine (NLM Chemicals) / Phosphoglycerate Dehydrogenase (NLM
                      Chemicals) / Glycine Hydroxymethyltransferase (NLM
                      Chemicals) / Transaminases (NLM Chemicals) / phosphoserine
                      aminotransferase (NLM Chemicals) / Phosphoric Monoester
                      Hydrolases (NLM Chemicals) / phosphoserine phosphatase (NLM
                      Chemicals) / L-Serine Dehydratase (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IBT-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB55},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK256},
      shelfmark    = {Biotechnology $\&$ Applied Microbiology / Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:16269752},
      pmc          = {pmc:PMC1287687},
      UT           = {WOS:000233225000083},
      doi          = {10.1128/AEM.71.11.7139-7144.2005},
      url          = {https://juser.fz-juelich.de/record/45521},
}