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@ARTICLE{Janzik:45605,
      author       = {Janzik, I. and Preiskowski, S. and Kneifel, H.},
      title        = {{O}zone has dramatic effects on the regulation of the
                      prechorismate pathway in tobacco ({N}icotiana tabacum
                      {L}.cv. {B}el {W}3)},
      journal      = {Planta},
      volume       = {223},
      issn         = {0032-0935},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {PreJuSER-45605},
      pages        = {20 - 27},
      year         = {2005},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {The accumulation of aromatic secondary metabolites is a
                      well-known element of the plant response to ozone. Most of
                      these metabolites are synthesized via the three aromatic
                      amino acids phenylalanine, tyrosine and tryptophan. Before
                      branching, the biosynthetic pathway to the three amino acids
                      shares seven enzymatic steps, called the prechorismate
                      pathway, catalysed by 3-deoxy-D:
                      -arabino-heptulosonate-7-phosphate (DAHP) synthase [EC
                      2.5.1.54], 3-dehydroquinate synthase [EC 4.2.3.4],
                      3-dehydroquinate dehydratase [EC 4.2.1.10]-shikimate
                      5-dehydrogenase [EC 1.1.1.25], shikimate kinase [EC
                      2.7.1.71], 5-enolpyruvylshikimate 3-phosphate synthase [EC
                      2.5.1.19] and chorismate synthase [EC 4.2.3.5]). We have
                      studied the transcript level of these enzymes and the
                      aromatic metabolite profile in the ozone sensitive tobacco
                      cultivar BelW3 (Nicotiana tabacum L. cv Bel W3), when
                      exposed to an acute ozone pulse (160 nl l(-1), 5 h).
                      Specific cDNA-fragments of the corresponding six genes were
                      isolated from tobacco Bel W3 and used as probes for
                      determining the expression of the prechorismate pathway
                      genes. The fully expanded leaves of ozone treated plants,
                      which developed symptoms like necrotic leaf spots and
                      accumulation of aromatic metabolites, showed a clear
                      induction of the shikimate pathway genes; indicating, that
                      this induction is linked to the development of the symptoms.
                      Distinct kinetics and magnitudes were observed in tobacco
                      leaves for the ozone dependent enhanced mRNA accumulation of
                      the aforementioned genes in BelW3. The strongest and
                      earliest induction due to ozone treatment could be observed
                      for DAHP synthase. An isoform-specific analysis of the
                      transcripts showed a strong induction on transcript level
                      only for one of three isoforms, which was followed by the
                      induction of the DAHP synthase also on protein level. The
                      different induction kinetics of the prechorismate pathway
                      genes indicate that their regulation in response to ozone
                      might be regulated by different signals, for example,
                      ethylene, reactive oxygen species or salicylic acid, which
                      also occur with different kinetics and thus may play
                      different roles in the plant response to ozone.},
      keywords     = {3-Deoxy-7-Phosphoheptulonate Synthase / Aldehyde-Lyases:
                      biosynthesis / Aldehyde-Lyases: genetics / Amino Acids,
                      Aromatic: biosynthesis / Blotting, Northern / Chorismic
                      Acid: metabolism / Chromatography, High Pressure Liquid /
                      DNA, Complementary / DNA, Plant / Enzyme Induction / Gene
                      Expression Regulation, Enzymologic / Gene Expression
                      Regulation, Plant / Ozone: pharmacology /
                      Phosphotransferases (Alcohol Group Acceptor): metabolism /
                      Plant Leaves / Tobacco: enzymology / Tobacco: genetics /
                      Tobacco: metabolism / Amino Acids, Aromatic (NLM Chemicals)
                      / DNA, Complementary (NLM Chemicals) / DNA, Plant (NLM
                      Chemicals) / Ozone (NLM Chemicals) / Chorismic Acid (NLM
                      Chemicals) / 3-Deoxy-7-Phosphoheptulonate Synthase (NLM
                      Chemicals) / Phosphotransferases (Alcohol Group Acceptor)
                      (NLM Chemicals) / shikimate kinase (NLM Chemicals) /
                      Aldehyde-Lyases (NLM Chemicals) / J (WoSType)},
      cin          = {ICG-III},
      ddc          = {580},
      cid          = {I:(DE-Juel1)VDB49},
      pnm          = {Chemie und Dynamik der Geo-Biosphäre},
      pid          = {G:(DE-Juel1)FUEK257},
      shelfmark    = {Plant Sciences},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:16078071},
      UT           = {WOS:000233725000003},
      doi          = {10.1007/s00425-005-0060-8},
      url          = {https://juser.fz-juelich.de/record/45605},
}