Home > Publications database > Ozone has dramatic effects on the regulation of the prechorismate pathway in tobacco (Nicotiana tabacum L.cv. Bel W3) > print

001     45605
005     20180209102221.0
024 7 _ |2 pmid
|a pmid:16078071
024 7 _ |2 DOI
|a 10.1007/s00425-005-0060-8
024 7 _ |2 WOS
|a WOS:000233725000003
037 _ _ |a PreJuSER-45605
041 _ _ |a eng
082 _ _ |a 580
084 _ _ |2 WoS
|a Plant Sciences
100 1 _ |a Janzik, I.
|b 0
|u FZJ
|0 P:(DE-Juel1)129338
245 _ _ |a Ozone has dramatic effects on the regulation of the prechorismate pathway in tobacco (Nicotiana tabacum L.cv. Bel W3)
260 _ _ |a Berlin
|b Springer
|c 2005
300 _ _ |a 20 - 27
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Planta
|x 0032-0935
|0 4992
|y 1
|v 223
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a The accumulation of aromatic secondary metabolites is a well-known element of the plant response to ozone. Most of these metabolites are synthesized via the three aromatic amino acids phenylalanine, tyrosine and tryptophan. Before branching, the biosynthetic pathway to the three amino acids shares seven enzymatic steps, called the prechorismate pathway, catalysed by 3-deoxy-D: -arabino-heptulosonate-7-phosphate (DAHP) synthase [EC 2.5.1.54], 3-dehydroquinate synthase [EC 4.2.3.4], 3-dehydroquinate dehydratase [EC 4.2.1.10]-shikimate 5-dehydrogenase [EC 1.1.1.25], shikimate kinase [EC 2.7.1.71], 5-enolpyruvylshikimate 3-phosphate synthase [EC 2.5.1.19] and chorismate synthase [EC 4.2.3.5]). We have studied the transcript level of these enzymes and the aromatic metabolite profile in the ozone sensitive tobacco cultivar BelW3 (Nicotiana tabacum L. cv Bel W3), when exposed to an acute ozone pulse (160 nl l(-1), 5 h). Specific cDNA-fragments of the corresponding six genes were isolated from tobacco Bel W3 and used as probes for determining the expression of the prechorismate pathway genes. The fully expanded leaves of ozone treated plants, which developed symptoms like necrotic leaf spots and accumulation of aromatic metabolites, showed a clear induction of the shikimate pathway genes; indicating, that this induction is linked to the development of the symptoms. Distinct kinetics and magnitudes were observed in tobacco leaves for the ozone dependent enhanced mRNA accumulation of the aforementioned genes in BelW3. The strongest and earliest induction due to ozone treatment could be observed for DAHP synthase. An isoform-specific analysis of the transcripts showed a strong induction on transcript level only for one of three isoforms, which was followed by the induction of the DAHP synthase also on protein level. The different induction kinetics of the prechorismate pathway genes indicate that their regulation in response to ozone might be regulated by different signals, for example, ethylene, reactive oxygen species or salicylic acid, which also occur with different kinetics and thus may play different roles in the plant response to ozone.
536 _ _ |a Chemie und Dynamik der Geo-Biosphäre
|c U01
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK257
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a 3-Deoxy-7-Phosphoheptulonate Synthase
650 _ 2 |2 MeSH
|a Aldehyde-Lyases: biosynthesis
650 _ 2 |2 MeSH
|a Aldehyde-Lyases: genetics
650 _ 2 |2 MeSH
|a Amino Acids, Aromatic: biosynthesis
650 _ 2 |2 MeSH
|a Blotting, Northern
650 _ 2 |2 MeSH
|a Chorismic Acid: metabolism
650 _ 2 |2 MeSH
|a Chromatography, High Pressure Liquid
650 _ 2 |2 MeSH
|a DNA, Complementary
650 _ 2 |2 MeSH
|a DNA, Plant
650 _ 2 |2 MeSH
|a Enzyme Induction
650 _ 2 |2 MeSH
|a Gene Expression Regulation, Enzymologic
650 _ 2 |2 MeSH
|a Gene Expression Regulation, Plant
650 _ 2 |2 MeSH
|a Ozone: pharmacology
650 _ 2 |2 MeSH
|a Phosphotransferases (Alcohol Group Acceptor): metabolism
650 _ 2 |2 MeSH
|a Plant Leaves
650 _ 2 |2 MeSH
|a Tobacco: enzymology
650 _ 2 |2 MeSH
|a Tobacco: genetics
650 _ 2 |2 MeSH
|a Tobacco: metabolism
650 _ 7 |0 0
|2 NLM Chemicals
|a Amino Acids, Aromatic
650 _ 7 |0 0
|2 NLM Chemicals
|a DNA, Complementary
650 _ 7 |0 0
|2 NLM Chemicals
|a DNA, Plant
650 _ 7 |0 10028-15-6
|2 NLM Chemicals
|a Ozone
650 _ 7 |0 617-12-9
|2 NLM Chemicals
|a Chorismic Acid
650 _ 7 |0 EC 2.5.1.54
|2 NLM Chemicals
|a 3-Deoxy-7-Phosphoheptulonate Synthase
650 _ 7 |0 EC 2.7.1.-
|2 NLM Chemicals
|a Phosphotransferases (Alcohol Group Acceptor)
650 _ 7 |0 EC 2.7.1.71
|2 NLM Chemicals
|a shikimate kinase
650 _ 7 |0 EC 4.1.2.-
|2 NLM Chemicals
|a Aldehyde-Lyases
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a prechorismate pathway
653 2 0 |2 Author
|a shikimate pathway
653 2 0 |2 Author
|a tobacco Bel W3
653 2 0 |2 Author
|a ozone
700 1 _ |a Preiskowski, S.
|b 1
|u FZJ
|0 P:(DE-Juel1)129386
700 1 _ |a Kneifel, H.
|b 2
|u FZJ
|0 P:(DE-Juel1)VDB4417
773 _ _ |a 10.1007/s00425-005-0060-8
|g Vol. 223, p. 20 - 27
|p 20 - 27
|q 223<20 - 27
|0 PERI:(DE-600)1463030-8
|t Planta
|v 223
|y 2005
|x 0032-0935
856 7 _ |u http://dx.doi.org/10.1007/s00425-005-0060-8
909 C O |o oai:juser.fz-juelich.de:45605
|p VDB
913 1 _ |k U01
|v Chemie und Dynamik der Geo-Biosphäre
|l Chemie und Dynamik der Geo-Biosphäre
|b Environment (Umwelt)
|0 G:(DE-Juel1)FUEK257
|x 0
914 1 _ |y 2005
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k ICG-III
|l Phytosphäre
|d 31.12.2006
|g ICG
|0 I:(DE-Juel1)VDB49
|x 0
970 _ _ |a VDB:(DE-Juel1)69966
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)IBG-2-20101118
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBG-2-20101118
981 _ _ |a I:(DE-Juel1)ICG-3-20090406

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