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000046012 0247_ $$2DOI$$a10.1111/j.1365-2958.2005.04560.x
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000046012 084__ $$2WoS$$aBiochemistry & Molecular Biology
000046012 084__ $$2WoS$$aMicrobiology
000046012 1001_ $$0P:(DE-HGF)0$$aAmoroso, G.$$b0
000046012 245__ $$aThe gene NCE103 (YNL036w) from Saccharomyces cerevisiae encodes a functional carbonic anhydrase and its transcription is regulated by the concentration of inorganic carbon in the medium
000046012 260__ $$aOxford [u.a.]$$bWiley-Blackwell$$c2005
000046012 300__ $$a549 - 558
000046012 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000046012 440_0 $$08441$$aMolecular Microbiology$$v56$$x0950-382X$$y2
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000046012 520__ $$aCarbonic anhydrase (CA) catalyses the rapid interconversion between CO(2) and HCO(3) (-). Despite its wide distribution among living organisms, the presence of CA in fungi has been controversially discussed. Using mass spectrometric analysis of (18)O exchange from doubly labelled CO(2), we were able to measure CA activity in intact cells of Saccharomyces cerevisiae. Intracellular CA activity was lacking in the Deltance103 mutant, indicating that NCE103 encodes a functional CA. This was proven by overexpressing and purification of the NCE103 gene product showing a specific activity of around 6900 units per mg protein. Interestingly, the in vivo CA activity was 10-20 times higher in cells grown on low inorganic carbon (Ci; air containing 0.035% CO(2)) than in high-Ci cells (grown on 5% CO(2)). The enhanced CA activity of low-Ci cells was inducible after transferring high-Ci cells to air. Northern blot analysis revealed that that expression of NCE103 is transcriptionally regulated by low Ci which was also demonstrated by fusing the NCE103 promoter to beta-galactosidase as a reporter gene. Inactivation of NCE103 results in a high CO(2) requiring mutant indicating that a functional CA is an important prerequisite for S. cerevisiae to grow under low-Ci conditions.
000046012 536__ $$0G:(DE-Juel1)FUEK257$$2G:(DE-HGF)$$aChemie und Dynamik der Geo-Biosphäre$$cU01$$x0
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000046012 650_2 $$2MeSH$$aCarbon: metabolism
000046012 650_2 $$2MeSH$$aCarbon: physiology
000046012 650_2 $$2MeSH$$aCarbonic Anhydrases: chemistry
000046012 650_2 $$2MeSH$$aCarbonic Anhydrases: classification
000046012 650_2 $$2MeSH$$aCarbonic Anhydrases: genetics
000046012 650_2 $$2MeSH$$aCarbonic Anhydrases: metabolism
000046012 650_2 $$2MeSH$$aCulture Media
000046012 650_2 $$2MeSH$$aGene Expression Regulation, Fungal
000046012 650_2 $$2MeSH$$aGenes, Fungal
000046012 650_2 $$2MeSH$$aSaccharomyces cerevisiae: enzymology
000046012 650_2 $$2MeSH$$aSaccharomyces cerevisiae: genetics
000046012 650_2 $$2MeSH$$aSaccharomyces cerevisiae: metabolism
000046012 650_2 $$2MeSH$$aTranscription, Genetic: physiology
000046012 650_7 $$00$$2NLM Chemicals$$aCulture Media
000046012 650_7 $$07440-44-0$$2NLM Chemicals$$aCarbon
000046012 650_7 $$0EC 4.2.1.1$$2NLM Chemicals$$aCarbonic Anhydrases
000046012 650_7 $$2WoSType$$aJ
000046012 7001_ $$0P:(DE-HGF)0$$aMorell-Avrahov, L.$$b1
000046012 7001_ $$0P:(DE-HGF)0$$aMüller, D.$$b2
000046012 7001_ $$0P:(DE-Juel1)VDB32478$$aKlug, K.$$b3$$uFZJ
000046012 7001_ $$0P:(DE-HGF)0$$aSültemeyer, D.$$b4
000046012 773__ $$0PERI:(DE-600)1501537-3$$a10.1111/j.1365-2958.2005.04560.x$$gVol. 56, p. 549 - 558$$p549 - 558$$q56<549 - 558$$tMolecular microbiology$$v56$$x0950-382X$$y2005
000046012 8567_ $$uhttp://dx.doi.org/10.1111/j.1365-2958.2005.04560.x
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