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@ARTICLE{Amoroso:46012,
      author       = {Amoroso, G. and Morell-Avrahov, L. and Müller, D. and
                      Klug, K. and Sültemeyer, D.},
      title        = {{T}he gene {NCE}103 ({YNL}036w) from {S}accharomyces
                      cerevisiae encodes a functional carbonic anhydrase and its
                      transcription is regulated by the concentration of inorganic
                      carbon in the medium},
      journal      = {Molecular microbiology},
      volume       = {56},
      issn         = {0950-382X},
      address      = {Oxford [u.a.]},
      publisher    = {Wiley-Blackwell},
      reportid     = {PreJuSER-46012},
      pages        = {549 - 558},
      year         = {2005},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Carbonic anhydrase (CA) catalyses the rapid interconversion
                      between CO(2) and HCO(3) (-). Despite its wide distribution
                      among living organisms, the presence of CA in fungi has been
                      controversially discussed. Using mass spectrometric analysis
                      of (18)O exchange from doubly labelled CO(2), we were able
                      to measure CA activity in intact cells of Saccharomyces
                      cerevisiae. Intracellular CA activity was lacking in the
                      Deltance103 mutant, indicating that NCE103 encodes a
                      functional CA. This was proven by overexpressing and
                      purification of the NCE103 gene product showing a specific
                      activity of around 6900 units per mg protein. Interestingly,
                      the in vivo CA activity was 10-20 times higher in cells
                      grown on low inorganic carbon (Ci; air containing $0.035\%$
                      CO(2)) than in high-Ci cells (grown on $5\%$ CO(2)). The
                      enhanced CA activity of low-Ci cells was inducible after
                      transferring high-Ci cells to air. Northern blot analysis
                      revealed that that expression of NCE103 is transcriptionally
                      regulated by low Ci which was also demonstrated by fusing
                      the NCE103 promoter to beta-galactosidase as a reporter
                      gene. Inactivation of NCE103 results in a high CO(2)
                      requiring mutant indicating that a functional CA is an
                      important prerequisite for S. cerevisiae to grow under
                      low-Ci conditions.},
      keywords     = {Carbon: metabolism / Carbon: physiology / Carbonic
                      Anhydrases: chemistry / Carbonic Anhydrases: classification
                      / Carbonic Anhydrases: genetics / Carbonic Anhydrases:
                      metabolism / Culture Media / Gene Expression Regulation,
                      Fungal / Genes, Fungal / Saccharomyces cerevisiae:
                      enzymology / Saccharomyces cerevisiae: genetics /
                      Saccharomyces cerevisiae: metabolism / Transcription,
                      Genetic: physiology / Culture Media (NLM Chemicals) / Carbon
                      (NLM Chemicals) / Carbonic Anhydrases (NLM Chemicals) / J
                      (WoSType)},
      cin          = {ICG-III},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB49},
      pnm          = {Chemie und Dynamik der Geo-Biosphäre},
      pid          = {G:(DE-Juel1)FUEK257},
      shelfmark    = {Biochemistry $\&$ Molecular Biology / Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:15813743},
      UT           = {WOS:000227897400020},
      doi          = {10.1111/j.1365-2958.2005.04560.x},
      url          = {https://juser.fz-juelich.de/record/46012},
}