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@ARTICLE{Schubert:46808,
author = {Schubert, D. and Staiger, J. F. and Cho, N. and Kötter, R.
and Zilles, K. and Luhmann, H. J.},
title = {{L}ayer-specific intracolumnar and transcolumnar functional
connectivity of layer {V} pyramidal cells in rat barrel
cortex},
journal = {The journal of neuroscience},
volume = {21},
issn = {0270-6474},
address = {Washington, DC},
publisher = {Soc.},
reportid = {PreJuSER-46808},
pages = {3580 - 3592},
year = {2001},
note = {Record converted from VDB: 12.11.2012},
abstract = {Layer V pyramidal cells in rat barrel cortex are considered
to play an important role in intracolumnar and transcolumnar
signal processing. However, the precise circuitry mediating
this processing is still incompletely understood. Here we
obtained detailed maps of excitatory and inhibitory synaptic
inputs onto the two major layer V pyramidal cell subtypes,
intrinsically burst spiking (IB) and regular spiking (RS)
cells, using a combination of caged glutamate photolysis,
whole- cell patch- clamp recording, and three- dimensional
reconstruction of biocytin- labeled cells. To excite
presynaptic neurons with laminar specificity, the release of
caged glutamate was calibrated and restricted to small areas
of 50 x 50 mum in all cortical layers and in at least two
neighboring barrel- related columns. IB cells received
intracolumnar excitatory input from all layers, with the
largest EPSP amplitudes originating from neurons in layers
IV and VI. Prominent transcolumnar excitatory inputs were
provided by presynaptic neurons also located in layers IV,
V, and VI of neighboring columns. Inhibitory inputs were
rare. In contrast, RS cells received distinct intracolumnar
inhibitory inputs, especially from layers II/ III and V.
Intracolumnar excitatory inputs to RS cells were prominent
from layers II- V, but relatively weak from layer VI.
Conspicuous transcolumnar excitatory inputs could be evoked
solely in layers IV and V. Our results show that layer V
pyramidal cells are synaptically driven by presynaptic
neurons located in every layer of the barrel cortex. RS
cells seem to be preferentially involved in intracolumnar
signal processing, whereas IB cells effectively integrate
excitatory inputs across several columns.},
keywords = {J (WoSType)},
cin = {IME},
ddc = {590},
cid = {I:(DE-Juel1)VDB54},
pnm = {Signaltransduktion},
pid = {G:(DE-Juel1)FUEK89},
shelfmark = {Neurosciences},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000168409400033},
url = {https://juser.fz-juelich.de/record/46808},
}