000046868 001__ 46868
000046868 005__ 20200402205958.0
000046868 0247_ $$2pmid$$apmid:11444865
000046868 0247_ $$2DOI$$a10.1006/bbrc.2001.5206
000046868 0247_ $$2WOS$$aWOS:000170020900046
000046868 037__ $$aPreJuSER-46868
000046868 041__ $$aeng
000046868 082__ $$a570
000046868 084__ $$2WoS$$aBiochemistry & Molecular Biology
000046868 084__ $$2WoS$$aBiophysics
000046868 1001_ $$0P:(DE-Juel1)VDB393$$aFischer, T.$$b0$$uFZJ
000046868 245__ $$aApplication of different surface plasmon resonance biosensor chips to monitor the interaction of the CaM-binding site of nitric oxide synthase I and calmodulin
000046868 260__ $$aOrlando, Fla.$$bAcademic Press$$c2001
000046868 300__ $$a463 - 469
000046868 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000046868 3367_ $$2DataCite$$aOutput Types/Journal article
000046868 3367_ $$00$$2EndNote$$aJournal Article
000046868 3367_ $$2BibTeX$$aARTICLE
000046868 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000046868 3367_ $$2DRIVER$$aarticle
000046868 440_0 $$0787$$aBiochemical and Biophysical Research Communications$$v285$$x0006-291X
000046868 500__ $$aRecord converted from VDB: 12.11.2012
000046868 520__ $$aSurface plasmon resonance biosensors depend on modified gold surfaces to allow immobilization of proteins or peptides for interaction analysis. We investigated sensor chip surfaces that differ in the geometry of the immobilization matrix: two contain a three-dimensional coupling matrix and two have a surface with immobilization sites on a two-dimensional plane. Properties of sensor chips were compared by studying the interaction of calmodulin with a peptide representing the calmodulin-binding site of nitric oxide synthase I. Apparent K(D) values were determined by three different procedures in order to apply tests for self-consistency. At low surface densities (5-8 fmol/mm(2)) on three of the four tested surfaces, estimated K(D) values were within one order of magnitude and similar to the value found in solution (K(D) = 1-3 nM). When immobilization densities were increased by one to two orders of magnitude, apparent association rate constants were less distorted on a flat carboxymethylated surface than on dextran-coated sensor chips.
000046868 536__ $$0G:(DE-Juel1)FUEK95$$2G:(DE-HGF)$$aZelluläre Signalverarbeitung$$c42.20.1$$x0
000046868 588__ $$aDataset connected to Web of Science, Pubmed
000046868 650_2 $$2MeSH$$aAmino Acid Sequence
000046868 650_2 $$2MeSH$$aAnimals
000046868 650_2 $$2MeSH$$aBinding Sites
000046868 650_2 $$2MeSH$$aBiosensing Techniques
000046868 650_2 $$2MeSH$$aCalmodulin: chemistry
000046868 650_2 $$2MeSH$$aCalmodulin: metabolism
000046868 650_2 $$2MeSH$$aKinetics
000046868 650_2 $$2MeSH$$aMolecular Sequence Data
000046868 650_2 $$2MeSH$$aNitric Oxide Synthase: chemistry
000046868 650_2 $$2MeSH$$aNitric Oxide Synthase: metabolism
000046868 650_2 $$2MeSH$$aNitric Oxide Synthase Type I
000046868 650_2 $$2MeSH$$aPeptides: chemistry
000046868 650_2 $$2MeSH$$aPeptides: metabolism
000046868 650_2 $$2MeSH$$aRats
000046868 650_2 $$2MeSH$$aSurface Plasmon Resonance: methods
000046868 650_7 $$00$$2NLM Chemicals$$aCalmodulin
000046868 650_7 $$00$$2NLM Chemicals$$aPeptides
000046868 650_7 $$0EC 1.14.13.39$$2NLM Chemicals$$aNitric Oxide Synthase
000046868 650_7 $$0EC 1.14.13.39$$2NLM Chemicals$$aNitric Oxide Synthase Type I
000046868 650_7 $$0EC 1.14.13.39$$2NLM Chemicals$$aNos1 protein, rat
000046868 650_7 $$2WoSType$$aJ
000046868 65320 $$2Author$$asurface plasmon resonance
000046868 65320 $$2Author$$abiosensor surfaces
000046868 65320 $$2Author$$aprotein-peptide interaction
000046868 65320 $$2Author$$acalmodulin
000046868 65320 $$2Author$$anitric oxide synthase
000046868 7001_ $$0P:(DE-HGF)0$$aBeyermann, M.$$b1
000046868 7001_ $$0P:(DE-Juel1)VDB789$$aKoch, K.-W.$$b2$$uFZJ
000046868 773__ $$0PERI:(DE-600)1461396-7$$a10.1006/bbrc.2001.5206$$gVol. 285, p. 463 - 469$$p463 - 469$$q285<463 - 469$$tBiochemical and biophysical research communications$$v285$$x0006-291X$$y2001
000046868 909CO $$ooai:juser.fz-juelich.de:46868$$pVDB
000046868 9131_ $$0G:(DE-Juel1)FUEK95$$bLebenswissenschaften$$k42.20.1$$lBiologische Informationsverarbeitung$$vZelluläre Signalverarbeitung$$x0
000046868 9141_ $$y2001
000046868 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000046868 9201_ $$0I:(DE-Juel1)VDB57$$d31.12.2006$$gIBI$$kIBI-1$$lZelluläre Signalverarbeitung$$x0
000046868 970__ $$aVDB:(DE-Juel1)975
000046868 980__ $$aVDB
000046868 980__ $$aConvertedRecord
000046868 980__ $$ajournal
000046868 980__ $$aI:(DE-Juel1)ICS-4-20110106
000046868 980__ $$aUNRESTRICTED
000046868 981__ $$aI:(DE-Juel1)IBI-1-20200312
000046868 981__ $$aI:(DE-Juel1)ICS-4-20110106