TY  - JOUR
AU  - Fischer, T.
AU  - Beyermann, M.
AU  - Koch, K.-W.
TI  - Application of different surface plasmon resonance biosensor chips to monitor the interaction of the CaM-binding site of nitric oxide synthase I and calmodulin
JO  - Biochemical and biophysical research communications
VL  - 285
SN  - 0006-291X
CY  - Orlando, Fla.
PB  - Academic Press
M1  - PreJuSER-46868
SP  - 463 - 469
PY  - 2001
N1  - Record converted from VDB: 12.11.2012
AB  - Surface plasmon resonance biosensors depend on modified gold surfaces to allow immobilization of proteins or peptides for interaction analysis. We investigated sensor chip surfaces that differ in the geometry of the immobilization matrix: two contain a three-dimensional coupling matrix and two have a surface with immobilization sites on a two-dimensional plane. Properties of sensor chips were compared by studying the interaction of calmodulin with a peptide representing the calmodulin-binding site of nitric oxide synthase I. Apparent K(D) values were determined by three different procedures in order to apply tests for self-consistency. At low surface densities (5-8 fmol/mm(2)) on three of the four tested surfaces, estimated K(D) values were within one order of magnitude and similar to the value found in solution (K(D) = 1-3 nM). When immobilization densities were increased by one to two orders of magnitude, apparent association rate constants were less distorted on a flat carboxymethylated surface than on dextran-coated sensor chips.
KW  - Amino Acid Sequence
KW  - Animals
KW  - Binding Sites
KW  - Biosensing Techniques
KW  - Calmodulin: chemistry
KW  - Calmodulin: metabolism
KW  - Kinetics
KW  - Molecular Sequence Data
KW  - Nitric Oxide Synthase: chemistry
KW  - Nitric Oxide Synthase: metabolism
KW  - Nitric Oxide Synthase Type I
KW  - Peptides: chemistry
KW  - Peptides: metabolism
KW  - Rats
KW  - Surface Plasmon Resonance: methods
KW  - Calmodulin (NLM Chemicals)
KW  - Peptides (NLM Chemicals)
KW  - Nitric Oxide Synthase (NLM Chemicals)
KW  - Nitric Oxide Synthase Type I (NLM Chemicals)
KW  - Nos1 protein, rat (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:11444865
UR  - <Go to ISI:>//WOS:000170020900046
DO  - DOI:10.1006/bbrc.2001.5206
UR  - https://juser.fz-juelich.de/record/46868
ER  -