TY  - JOUR
AU  - Fabiani, E.
AU  - Stadler, A.M.
AU  - Madern, D.
AU  - Hirai, M.
AU  - Zaccai, G.
TI  - Dynamics of apo-myoglobin in the alpha-to-beta transition and of partially unfolded aggregated protein
JO  - European biophysics journal
VL  - 38
SN  - 0175-7571
CY  - Berlin
PB  - Springer
M1  - PreJuSER-4699
SP  - 237 - 244
PY  - 2009
N1  - The authors acknowledge the IN13 and IRIS staffs for their support during the experiment at ILL and ISIS facilities. Thanks are due to Dr. Felix Fernandez-Alonso and Dr. Franz Demmel for helpful discussions. Technical and financial support from the ILL and ISIS facilities is gratefully acknowledged. The WAXS measurement was done under the approval of the JASRI Program Advisory Committee ( Proposal #2006A1339). M. Tehei acknowledges the ILL, the AINSE, the CNRS and the Institut de Biologie Structurale (UMR 5075) for financial support of this work.
AB  - Changes of molecular dynamics in the alpha-to-beta transition associated with amyloid fibril formation were explored on apomyoglobin (ApoMb) as a model system. Circular dichroism, neutron and X-ray scattering experiments were performed as a function of temperature on the protein, at different solvent conditions. A significant change in molecular dynamics was observed at the alpha-to-beta transition at about 55 degrees C, indicating a more resilient high temperature beta structure phase. A similar effect at approximately the same temperature was observed in holo-myoglobin, associated with partial unfolding and protein aggregation. A study in a wide temperature range between 20 and 360 K revealed that a dynamical transition at about 200 K for motions in the 50 ps time scale exists also for a hydrated powder of heat-denatured aggregated ApoMb.
KW  - Amyloidosis: physiopathology
KW  - Apoproteins: chemistry
KW  - Circular Dichroism
KW  - Crystallography, X-Ray
KW  - Models, Molecular
KW  - Myoglobin: chemistry
KW  - Neutron Diffraction
KW  - Pharmaceutical Solutions
KW  - Protein Folding
KW  - Protein Multimerization
KW  - Protein Structure, Tertiary
KW  - Temperature
KW  - Thermodynamics
KW  - Apoproteins (NLM Chemicals)
KW  - Myoglobin (NLM Chemicals)
KW  - Pharmaceutical Solutions (NLM Chemicals)
KW  - apomyoglobin (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:18853152
UR  - <Go to ISI:>//WOS:000262671600010
DO  - DOI:10.1007/s00249-008-0375-z
UR  - https://juser.fz-juelich.de/record/4699
ER  -