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@ARTICLE{Fabiani:4699,
author = {Fabiani, E. and Stadler, A.M. and Madern, D. and Hirai, M.
and Zaccai, G.},
title = {{D}ynamics of apo-myoglobin in the alpha-to-beta transition
and of partially unfolded aggregated protein},
journal = {European biophysics journal},
volume = {38},
issn = {0175-7571},
address = {Berlin},
publisher = {Springer},
reportid = {PreJuSER-4699},
pages = {237 - 244},
year = {2009},
note = {The authors acknowledge the IN13 and IRIS staffs for their
support during the experiment at ILL and ISIS facilities.
Thanks are due to Dr. Felix Fernandez-Alonso and Dr. Franz
Demmel for helpful discussions. Technical and financial
support from the ILL and ISIS facilities is gratefully
acknowledged. The WAXS measurement was done under the
approval of the JASRI Program Advisory Committee ( Proposal
#2006A1339). M. Tehei acknowledges the ILL, the AINSE, the
CNRS and the Institut de Biologie Structurale (UMR 5075) for
financial support of this work.},
abstract = {Changes of molecular dynamics in the alpha-to-beta
transition associated with amyloid fibril formation were
explored on apomyoglobin (ApoMb) as a model system. Circular
dichroism, neutron and X-ray scattering experiments were
performed as a function of temperature on the protein, at
different solvent conditions. A significant change in
molecular dynamics was observed at the alpha-to-beta
transition at about 55 degrees C, indicating a more
resilient high temperature beta structure phase. A similar
effect at approximately the same temperature was observed in
holo-myoglobin, associated with partial unfolding and
protein aggregation. A study in a wide temperature range
between 20 and 360 K revealed that a dynamical transition at
about 200 K for motions in the 50 ps time scale exists also
for a hydrated powder of heat-denatured aggregated ApoMb.},
keywords = {Amyloidosis: physiopathology / Apoproteins: chemistry /
Circular Dichroism / Crystallography, X-Ray / Models,
Molecular / Myoglobin: chemistry / Neutron Diffraction /
Pharmaceutical Solutions / Protein Folding / Protein
Multimerization / Protein Structure, Tertiary / Temperature
/ Thermodynamics / Apoproteins (NLM Chemicals) / Myoglobin
(NLM Chemicals) / Pharmaceutical Solutions (NLM Chemicals) /
apomyoglobin (NLM Chemicals) / J (WoSType)},
cin = {ISB-2},
ddc = {570},
cid = {I:(DE-Juel1)ISB-2-20090406},
pnm = {Programm Biosoft},
pid = {G:(DE-Juel1)FUEK443},
shelfmark = {Biophysics},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:18853152},
UT = {WOS:000262671600010},
doi = {10.1007/s00249-008-0375-z},
url = {https://juser.fz-juelich.de/record/4699},
}