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@ARTICLE{Artmann:4701,
      author       = {Artmann, G.M. and Digel, I. and Zerlin, K.F. and
                      Maggakis-Kelemen, Ch. and Linder, Pt. and Porst, D. and
                      Stadler, A.M. and Kayser, P. and Dikta, G. and Temiz
                      Artmann, A.},
      title        = {{H}emoglobin senses body temperature},
      journal      = {European biophysics journal},
      volume       = {38},
      issn         = {0175-7571},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {PreJuSER-4701},
      pages        = {589 - 600},
      year         = {2009},
      note         = {This work was supported by the Ministry of Innovation,
                      Science, Research and Technology of the State of North
                      Rhine-Westphalia to G. M.},
      abstract     = {When aspirating human red blood cells (RBCs) into 1.3 mum
                      pipettes (DeltaP = -2.3 kPa), a transition from blocking the
                      pipette below a critical temperature T(c) = 36.3 +/- 0.3
                      degrees C to passing it above the T(c) occurred
                      (micropipette passage transition). With a 1.1 mum pipette no
                      passage was seen which enabled RBC volume measurements also
                      above T(c). With increasing temperature RBCs lost volume
                      significantly faster below than above a T(c) = 36.4 +/- 0.7
                      (volume transition). Colloid osmotic pressure (COP)
                      measurements of RBCs in autologous plasma (25 degrees C < or
                      = T < or = 39.5 degrees C) showed a T (c) at 37.1 +/- 0.2
                      degrees C above which the COP rapidly decreased (COP
                      transition). In NMR T(1)-relaxation time measurements, the
                      T(1) of RBCs in autologous plasma changed from a linear (r =
                      0.99) increment below T(c) = 37 +/- 1 degrees C at a rate of
                      0.023 s/K into zero slope above T(c) (RBC T(1) transition).
                      In conclusion: An amorphous hemoglobin-water gel formed in
                      the spherical trail, the residual partial sphere of the
                      aspirated RBC. At T(c), a sudden fluidization of the gel
                      occurs. All changes mentioned above happen at a distinct
                      T(c) close to body temperature. The T(c) is moved +0.8
                      degrees C to higher temperatures when a D(2)O buffer is
                      used. We suggest a mechanism similar to a "glass transition"
                      or a "colloidal phase transition". At T(c), the stabilizing
                      Hb bound water molecules reach a threshold number enabling a
                      partial Hb unfolding. Thus, Hb senses body temperature which
                      must be inscribed in the primary structure of hemoglobin and
                      possibly other proteins.},
      keywords     = {Body Temperature / Erythrocyte Volume / Hemoglobins:
                      chemistry / Hemoglobins: metabolism / Humans / Magnetic
                      Resonance Spectroscopy / Osmotic Pressure / Phase Transition
                      / Temperature / Water: metabolism / Hemoglobins (NLM
                      Chemicals) / Water (NLM Chemicals) / J (WoSType)},
      cin          = {ISB-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ISB-2-20090406},
      pnm          = {Programm Biosoft},
      pid          = {G:(DE-Juel1)FUEK443},
      shelfmark    = {Biophysics},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:19238378},
      UT           = {WOS:000265917300005},
      doi          = {10.1007/s00249-009-0410-8},
      url          = {https://juser.fz-juelich.de/record/4701},
}