TY  - JOUR
AU  - Stadler, A.M.
AU  - Digel, I.
AU  - Embs, J.P.
AU  - Unruh, T.
AU  - Zaccai, G.
AU  - Büldt, G.
AU  - Artmann, G.
TI  - From Powder to Solution: Hemoglobin Dynamics Correlated to Body Temperature
JO  - Biophysical journal
VL  - 96
SN  - 0006-3495
CY  - New York, NY
PB  - Rockefeller Univ. Press
M1  - PreJuSER-4704
SP  - 5073 - 5081
PY  - 2009
N1  - This work was supported by the European Commission under the 6th Framework Programme through the Key Action: Strengthening the European Research Area, Research Infrastructures (RII3-CT-2003-505925), the Institut Laue-Langevin (M.T.), and the AINSE (M.T.).
AB  - A transition in hemoglobin (Hb), involving partial unfolding and aggregation, has been shown previously by various biophysical methods. The correlation between the transition temperature and body temperature for Hb from different species, suggested that it might be significant for biological function. To focus on such biologically relevant human Hb dynamics, we studied the protein internal picosecond motions as a response to hydration, by elastic and quasielastic neutron scattering. Rates of fast diffusive motions were found to be significantly enhanced with increasing hydration from fully hydrated powder to concentrated Hb solution. In concentrated protein solution, the data showed that amino acid side chains can explore larger volumes above body temperature than expected from normal temperature dependence. The body temperature transition in protein dynamics was absent in fully hydrated powder, indicating that picosecond protein dynamics responsible for the transition is activated only at a sufficient level of hydration. A collateral result from the study is that fully hydrated protein powder samples do not accurately describe all aspects of protein picosecond dynamics that might be necessary for biological function.
KW  - Body Temperature
KW  - Elasticity
KW  - Hemoglobins: chemistry
KW  - Humans
KW  - Neutrons
KW  - Powders
KW  - Solutions
KW  - Water: chemistry
KW  - Hemoglobins (NLM Chemicals)
KW  - Powders (NLM Chemicals)
KW  - Solutions (NLM Chemicals)
KW  - Water (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19527667
C2  - pmc:PMC2712052
UR  - <Go to ISI:>//WOS:000267194600032
DO  - DOI:10.1016/j.bpj.2009.03.043
UR  - https://juser.fz-juelich.de/record/4704
ER  -