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@ARTICLE{Gabriel:47188,
author = {Gabriel, F. and Giger, W. and Günther, K. and Kohler,
H.-P.},
title = {{D}ifferential degradation of nonylphenol isomers by
{S}phingomonas xenophaga {B}ayram},
journal = {Applied and environmental microbiology},
volume = {71},
issn = {0099-2240},
address = {Washington, DC [u.a.]},
publisher = {Soc.},
reportid = {PreJuSER-47188},
pages = {1123 - 1129},
year = {2005},
note = {Record converted from VDB: 12.11.2012},
abstract = {Sphingomonas xenophaga Bayram, isolated from the activated
sludge of a municipal wastewater treatment plant, was able
to utilize 4-(1-ethyl-1,4-dimethylpentyl)phenol, one of the
main isomers of technical nonylphenol mixtures, as a sole
carbon and energy source. The isolate degraded 1 mg of
4-(1-ethyl-1,4-dimethylpentyl)phenol/ml in minimal medium
within 1 week. Growth experiments with five nonylphenol
isomers showed that the three isomers with quaternary
benzylic carbon atoms [(1,1,2,4-tetramethylpentyl)phenol,
4-(1-ethyl-1,4-dimethylpentyl)phenol, and
4-(1,1-dimethylheptyl)phenol] served as growth substrates,
whereas the isomers containing one or two hydrogen atoms in
the benzylic position [4-(1-methyloctyl)phenol and
4-n-nonylphenol] did not. However, when the isomers were
incubated as a mixture, all were degraded to a certain
degree. Differential degradation was clearly evident, as
isomers with more highly branched alkyl side chains were
degraded much faster than the others. Furthermore, the C9
alcohols 2,3,5-trimethylhexan-2-ol, 3,6-dimethylheptan-3-ol,
and 2-methyloctan-2-ol, derived from the three nonylphenol
isomers with quaternary benzylic carbon atoms, were detected
in the culture fluid by gas chromatography-mass
spectrometry, but no analogous metabolites could be found
originating from 4-(1-methyloctyl)phenol and
4-n-nonylphenol. We propose that 4-(1-methyloctyl)phenol and
4-n-nonylphenol were cometabolically transformed in the
growth experiments with the mixture but that, unlike the
other isomers, they did not participate in the reactions
leading to the detachment of the alkyl moiety. This
hypothesis was corroborated by the observed accumulation in
the culture fluid of an as yet unidentified metabolite
derived from 4-(1-methyloctyl)phenol.},
keywords = {Alcohols: chemistry / Alcohols: metabolism /
Biodegradation, Environmental / Gas Chromatography-Mass
Spectrometry / Isomerism / Molecular Structure / Phenols:
chemistry / Phenols: metabolism / Sewage: microbiology /
Sphingomonas: isolation $\&$ purification / Sphingomonas:
metabolism / Alcohols (NLM Chemicals) / Phenols (NLM
Chemicals) / Sewage (NLM Chemicals) / nonylphenol (NLM
Chemicals) / J (WoSType)},
cin = {ICG-III},
ddc = {570},
cid = {I:(DE-Juel1)VDB49},
pnm = {Chemie und Dynamik der Geo-Biosphäre},
pid = {G:(DE-Juel1)FUEK257},
shelfmark = {Biotechnology $\&$ Applied Microbiology / Microbiology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:15746308},
pmc = {pmc:PMC1065174},
UT = {WOS:000227702900001},
doi = {10.1128/AEM.71.3.1123-1129.2005},
url = {https://juser.fz-juelich.de/record/47188},
}