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000047496 084__ $$2WoS$$aPlant Sciences
000047496 1001_ $$0P:(DE-HGF)0$$aGould, N.$$b0
000047496 245__ $$aPhloem hydrostatic pressure relates to solute loading rate: a direct test of Münch hypothesis
000047496 260__ $$aCollingwood, Victoria$$bCSIRO Publ.$$c2005
000047496 300__ $$a1019 - 1026
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000047496 440_0 $$09141$$aFunctional Plant Biology$$v32$$x1445-4408$$y11
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000047496 520__ $$aAccording to the Munch hypothesis, a flow of solution through the sieve tubes is driven by a hydrostatic pressure difference between the source ( or collection) phloem and the sink ( or release) phloem. A high hydrostatic pressure is maintained in the collection phloem by the active uptake of sugar and other solutes, with a concomitant inflow of water. A lower pressure is maintained in the release phloem through solute unloading. In this work we directly test the role of solute uptake in creating the hydrostatic pressure associated with phloem flow. Solute loading into the phloem of mature leaves of barley and sow thistle was reduced by replacing the air supply with nitrogen gas. Hydrostatic pressure in adjacent sieve elements was measured with a sieve-element pressure probe, a cell pressure probe glued to the exuding stylet of aphids that had been feeding from the phloem. Sieve element sap was sampled by aphid stylectomy; sap osmotic pressure was determined by picolitre osmometry and its sugar concentration by enzyme-linked fluorescence assays. Samples were taken with a time resolution of similar to 2 - 3 min. In accordance with Munch's proposal a drop in osmotic and hydrostatic pressure in the source phloem following treatment of the source leaf with N-2 was observed. A decrease in sugar concentration was the major contributor to the change in osmotic pressure. By observing these variables at a time resolution of minutes we have direct observation of the predictions of Munch.
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000047496 65320 $$2Author$$aanoxia
000047496 65320 $$2Author$$aaphid stylectomy
000047496 65320 $$2Author$$aMunch hypothesis
000047496 65320 $$2Author$$aphloem loading
000047496 65320 $$2Author$$aphloem pressure probe
000047496 7001_ $$0P:(DE-HGF)0$$aThorpe, M. R.$$b1
000047496 7001_ $$0P:(DE-HGF)0$$aKoroleva, O.$$b2
000047496 7001_ $$0P:(DE-Juel1)VDB23736$$aMinchin, P. E. H.$$b3$$uFZJ
000047496 773__ $$0PERI:(DE-600)1496158-1$$a10.1071/FP05036$$gVol. 32, p. 1019 - 1026$$p1019 - 1026$$q32<1019 - 1026$$tFunctional plant biology$$v32$$x1445-4408$$y2005
000047496 8567_ $$uhttp://dx.doi.org/10.1071/FP05036
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