Home > Publications database > Phloem hydrostatic pressure relates to solute loading rate: a direct test of Münch hypothesis > print

001     47496
005     20180210132114.0
024 7 _ |2 DOI
|a 10.1071/FP05036
024 7 _ |2 WOS
|a WOS:000232856600005
037 _ _ |a PreJuSER-47496
041 _ _ |a eng
082 _ _ |a 580
084 _ _ |2 WoS
|a Plant Sciences
100 1 _ |a Gould, N.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a Phloem hydrostatic pressure relates to solute loading rate: a direct test of Münch hypothesis
260 _ _ |a Collingwood, Victoria
|b CSIRO Publ.
|c 2005
300 _ _ |a 1019 - 1026
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Functional Plant Biology
|x 1445-4408
|0 9141
|y 11
|v 32
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a According to the Munch hypothesis, a flow of solution through the sieve tubes is driven by a hydrostatic pressure difference between the source ( or collection) phloem and the sink ( or release) phloem. A high hydrostatic pressure is maintained in the collection phloem by the active uptake of sugar and other solutes, with a concomitant inflow of water. A lower pressure is maintained in the release phloem through solute unloading. In this work we directly test the role of solute uptake in creating the hydrostatic pressure associated with phloem flow. Solute loading into the phloem of mature leaves of barley and sow thistle was reduced by replacing the air supply with nitrogen gas. Hydrostatic pressure in adjacent sieve elements was measured with a sieve-element pressure probe, a cell pressure probe glued to the exuding stylet of aphids that had been feeding from the phloem. Sieve element sap was sampled by aphid stylectomy; sap osmotic pressure was determined by picolitre osmometry and its sugar concentration by enzyme-linked fluorescence assays. Samples were taken with a time resolution of similar to 2 - 3 min. In accordance with Munch's proposal a drop in osmotic and hydrostatic pressure in the source phloem following treatment of the source leaf with N-2 was observed. A decrease in sugar concentration was the major contributor to the change in osmotic pressure. By observing these variables at a time resolution of minutes we have direct observation of the predictions of Munch.
536 _ _ |a Chemie und Dynamik der Geo-Biosphäre
|c U01
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK257
|x 0
588 _ _ |a Dataset connected to Web of Science
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a anoxia
653 2 0 |2 Author
|a aphid stylectomy
653 2 0 |2 Author
|a Munch hypothesis
653 2 0 |2 Author
|a phloem loading
653 2 0 |2 Author
|a phloem pressure probe
700 1 _ |a Thorpe, M. R.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Koroleva, O.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Minchin, P. E. H.
|b 3
|u FZJ
|0 P:(DE-Juel1)VDB23736
773 _ _ |a 10.1071/FP05036
|g Vol. 32, p. 1019 - 1026
|p 1019 - 1026
|q 32<1019 - 1026
|0 PERI:(DE-600)1496158-1
|t Functional plant biology
|v 32
|y 2005
|x 1445-4408
856 7 _ |u http://dx.doi.org/10.1071/FP05036
909 C O |o oai:juser.fz-juelich.de:47496
|p VDB
913 1 _ |k U01
|v Chemie und Dynamik der Geo-Biosphäre
|l Chemie und Dynamik der Geo-Biosphäre
|b Environment (Umwelt)
|0 G:(DE-Juel1)FUEK257
|x 0
914 1 _ |y 2005
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k ICG-III
|l Phytosphäre
|d 31.12.2006
|g ICG
|0 I:(DE-Juel1)VDB49
|x 0
970 _ _ |a VDB:(DE-Juel1)74930
980 _ _ |a VDB
980 _ _ |a ConvertedRecord
980 _ _ |a journal
980 _ _ |a I:(DE-Juel1)IBG-2-20101118
980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBG-2-20101118
981 _ _ |a I:(DE-Juel1)ICG-3-20090406

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