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000047527 0247_ $$2DOI$$a10.1128/JB.187.22.7639-7646.2005
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000047527 084__ $$2WoS$$aMicrobiology
000047527 1001_ $$0P:(DE-Juel1)144031$$aMarienhagen, J.$$b0$$uFZJ
000047527 245__ $$aFunctional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum
000047527 260__ $$aWashington, DC$$bSoc.$$c2005
000047527 300__ $$a7639 - 7646
000047527 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000047527 440_0 $$03082$$aJournal of Bacteriology$$v187$$x0021-9193$$y22
000047527 500__ $$aRecord converted from VDB: 12.11.2012
000047527 520__ $$aTwenty putative aminotransferase (AT) proteins of Corynebacterium glutamicum, or rather pyridoxal-5'-phosphate (PLP)-dependent enzymes, were isolated and assayed among others with L-glutamate, L-aspartate, and L-alanine as amino donors and a number of 2-oxo-acids as amino acceptors. One outstanding AT identified is AlaT, which has a broad amino donor specificity utilizing (in the order of preference) L-glutamate > 2-aminobutyrate > L-aspartate with pyruvate as acceptor. Another AT is AvtA, which utilizes L-alanine to aminate 2-oxo-isovalerate, the L-valine precursor, and 2-oxo-butyrate. A second AT active with the L-valine precursor and that of the other two branched-chain amino acids, too, is IlvE, and both enzyme activities overlap partially in vivo, as demonstrated by the analysis of deletion mutants. Also identified was AroT, the aromatic AT, and this and IlvE were shown to have comparable activities with phenylpyruvate, thus demonstrating the relevance of both ATs for L-phenylalanine synthesis. We also assessed the activity of two PLP-containing cysteine desulfurases, supplying a persulfide intermediate. One of them is SufS, which assists in the sulfur transfer pathway for the Fe-S cluster assembly. Together with the identification of further ATs and the additional analysis of deletion mutants, this results in an overview of the ATs within an organism that may not have been achieved thus far.
000047527 536__ $$0G:(DE-Juel1)FUEK256$$2G:(DE-HGF)$$aBiotechnologie$$cL02$$x0
000047527 588__ $$aDataset connected to Web of Science, Pubmed
000047527 650_2 $$2MeSH$$aAlanine: metabolism
000047527 650_2 $$2MeSH$$aAspartic Acid: metabolism
000047527 650_2 $$2MeSH$$aBacterial Proteins: genetics
000047527 650_2 $$2MeSH$$aBacterial Proteins: isolation & purification
000047527 650_2 $$2MeSH$$aBacterial Proteins: metabolism
000047527 650_2 $$2MeSH$$aButyric Acids: metabolism
000047527 650_2 $$2MeSH$$aCarbon-Sulfur Lyases: metabolism
000047527 650_2 $$2MeSH$$aCorynebacterium glutamicum: enzymology
000047527 650_2 $$2MeSH$$aCorynebacterium glutamicum: genetics
000047527 650_2 $$2MeSH$$aGene Deletion
000047527 650_2 $$2MeSH$$aGlutamic Acid: metabolism
000047527 650_2 $$2MeSH$$aKeto Acids: metabolism
000047527 650_2 $$2MeSH$$aPhenylpyruvic Acids: metabolism
000047527 650_2 $$2MeSH$$aPyruvic Acid: metabolism
000047527 650_2 $$2MeSH$$aSubstrate Specificity
000047527 650_2 $$2MeSH$$aTransaminases: genetics
000047527 650_2 $$2MeSH$$aTransaminases: isolation & purification
000047527 650_2 $$2MeSH$$aTransaminases: metabolism
000047527 650_7 $$00$$2NLM Chemicals$$aBacterial Proteins
000047527 650_7 $$00$$2NLM Chemicals$$aButyric Acids
000047527 650_7 $$00$$2NLM Chemicals$$aKeto Acids
000047527 650_7 $$00$$2NLM Chemicals$$aPhenylpyruvic Acids
000047527 650_7 $$0127-17-3$$2NLM Chemicals$$aPyruvic Acid
000047527 650_7 $$01460-34-0$$2NLM Chemicals$$aalpha-keto-beta-methylvaleric acid
000047527 650_7 $$0156-06-9$$2NLM Chemicals$$aphenylpyruvic acid
000047527 650_7 $$056-41-7$$2NLM Chemicals$$aAlanine
000047527 650_7 $$056-84-8$$2NLM Chemicals$$aAspartic Acid
000047527 650_7 $$056-86-0$$2NLM Chemicals$$aGlutamic Acid
000047527 650_7 $$0600-18-0$$2NLM Chemicals$$aalpha-ketobutyric acid
000047527 650_7 $$0759-05-7$$2NLM Chemicals$$aalpha-ketoisovalerate
000047527 650_7 $$0816-66-0$$2NLM Chemicals$$aalpha-ketoisocaproic acid
000047527 650_7 $$0EC 2.6.1.-$$2NLM Chemicals$$aTransaminases
000047527 650_7 $$0EC 4.4.-$$2NLM Chemicals$$aCarbon-Sulfur Lyases
000047527 650_7 $$0EC 4.4.1.-$$2NLM Chemicals$$acysteine desulfurase
000047527 650_7 $$2WoSType$$aJ
000047527 7001_ $$0P:(DE-Juel1)VDB2921$$aKennerknecht, N.$$b1$$uFZJ
000047527 7001_ $$0P:(DE-Juel1)128985$$aSahm, H.$$b2$$uFZJ
000047527 7001_ $$0P:(DE-Juel1)VDB57928$$aEggeling, L.$$b3$$uFZJ
000047527 773__ $$0PERI:(DE-600)1481988-0$$a10.1128/JB.187.22.7639-7646.2005$$gVol. 187, p. 7639 - 7646$$p7639 - 7646$$q187<7639 - 7646$$tJournal of bacteriology$$v187$$x0021-9193$$y2005
000047527 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1280304
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