Home > Publications database > Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum > print

001     47527
005     20200423204233.0
024 7 _ |a pmid:16267288
|2 pmid
024 7 _ |a pmc:PMC1280304
|2 pmc
024 7 _ |a 10.1128/JB.187.22.7639-7646.2005
|2 DOI
024 7 _ |a WOS:000233400200011
|2 WOS
024 7 _ |a 2128/2426
|2 Handle
024 7 _ |a altmetric:21814223
|2 altmetric
037 _ _ |a PreJuSER-47527
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Microbiology
100 1 _ |a Marienhagen, J.
|b 0
|u FZJ
|0 P:(DE-Juel1)144031
245 _ _ |a Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum
260 _ _ |a Washington, DC
|b Soc.
|c 2005
300 _ _ |a 7639 - 7646
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
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336 7 _ |a ARTICLE
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336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Journal of Bacteriology
|x 0021-9193
|0 3082
|y 22
|v 187
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Twenty putative aminotransferase (AT) proteins of Corynebacterium glutamicum, or rather pyridoxal-5'-phosphate (PLP)-dependent enzymes, were isolated and assayed among others with L-glutamate, L-aspartate, and L-alanine as amino donors and a number of 2-oxo-acids as amino acceptors. One outstanding AT identified is AlaT, which has a broad amino donor specificity utilizing (in the order of preference) L-glutamate > 2-aminobutyrate > L-aspartate with pyruvate as acceptor. Another AT is AvtA, which utilizes L-alanine to aminate 2-oxo-isovalerate, the L-valine precursor, and 2-oxo-butyrate. A second AT active with the L-valine precursor and that of the other two branched-chain amino acids, too, is IlvE, and both enzyme activities overlap partially in vivo, as demonstrated by the analysis of deletion mutants. Also identified was AroT, the aromatic AT, and this and IlvE were shown to have comparable activities with phenylpyruvate, thus demonstrating the relevance of both ATs for L-phenylalanine synthesis. We also assessed the activity of two PLP-containing cysteine desulfurases, supplying a persulfide intermediate. One of them is SufS, which assists in the sulfur transfer pathway for the Fe-S cluster assembly. Together with the identification of further ATs and the additional analysis of deletion mutants, this results in an overview of the ATs within an organism that may not have been achieved thus far.
536 _ _ |a Biotechnologie
|c L02
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK256
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Alanine: metabolism
650 _ 2 |2 MeSH
|a Aspartic Acid: metabolism
650 _ 2 |2 MeSH
|a Bacterial Proteins: genetics
650 _ 2 |2 MeSH
|a Bacterial Proteins: isolation & purification
650 _ 2 |2 MeSH
|a Bacterial Proteins: metabolism
650 _ 2 |2 MeSH
|a Butyric Acids: metabolism
650 _ 2 |2 MeSH
|a Carbon-Sulfur Lyases: metabolism
650 _ 2 |2 MeSH
|a Corynebacterium glutamicum: enzymology
650 _ 2 |2 MeSH
|a Corynebacterium glutamicum: genetics
650 _ 2 |2 MeSH
|a Gene Deletion
650 _ 2 |2 MeSH
|a Glutamic Acid: metabolism
650 _ 2 |2 MeSH
|a Keto Acids: metabolism
650 _ 2 |2 MeSH
|a Phenylpyruvic Acids: metabolism
650 _ 2 |2 MeSH
|a Pyruvic Acid: metabolism
650 _ 2 |2 MeSH
|a Substrate Specificity
650 _ 2 |2 MeSH
|a Transaminases: genetics
650 _ 2 |2 MeSH
|a Transaminases: isolation & purification
650 _ 2 |2 MeSH
|a Transaminases: metabolism
650 _ 7 |0 0
|2 NLM Chemicals
|a Bacterial Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Butyric Acids
650 _ 7 |0 0
|2 NLM Chemicals
|a Keto Acids
650 _ 7 |0 0
|2 NLM Chemicals
|a Phenylpyruvic Acids
650 _ 7 |0 127-17-3
|2 NLM Chemicals
|a Pyruvic Acid
650 _ 7 |0 1460-34-0
|2 NLM Chemicals
|a alpha-keto-beta-methylvaleric acid
650 _ 7 |0 156-06-9
|2 NLM Chemicals
|a phenylpyruvic acid
650 _ 7 |0 56-41-7
|2 NLM Chemicals
|a Alanine
650 _ 7 |0 56-84-8
|2 NLM Chemicals
|a Aspartic Acid
650 _ 7 |0 56-86-0
|2 NLM Chemicals
|a Glutamic Acid
650 _ 7 |0 600-18-0
|2 NLM Chemicals
|a alpha-ketobutyric acid
650 _ 7 |0 759-05-7
|2 NLM Chemicals
|a alpha-ketoisovalerate
650 _ 7 |0 816-66-0
|2 NLM Chemicals
|a alpha-ketoisocaproic acid
650 _ 7 |0 EC 2.6.1.-
|2 NLM Chemicals
|a Transaminases
650 _ 7 |0 EC 4.4.-
|2 NLM Chemicals
|a Carbon-Sulfur Lyases
650 _ 7 |0 EC 4.4.1.-
|2 NLM Chemicals
|a cysteine desulfurase
650 _ 7 |a J
|2 WoSType
700 1 _ |a Kennerknecht, N.
|b 1
|u FZJ
|0 P:(DE-Juel1)VDB2921
700 1 _ |a Sahm, H.
|b 2
|u FZJ
|0 P:(DE-Juel1)128985
700 1 _ |a Eggeling, L.
|b 3
|u FZJ
|0 P:(DE-Juel1)VDB57928
773 _ _ |a 10.1128/JB.187.22.7639-7646.2005
|g Vol. 187, p. 7639 - 7646
|p 7639 - 7646
|q 187<7639 - 7646
|0 PERI:(DE-600)1481988-0
|t Journal of bacteriology
|v 187
|y 2005
|x 0021-9193
856 7 _ |2 Pubmed Central
|u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1280304
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