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@ARTICLE{Mayer:47540,
author = {Mayer, D. and Ataka, K. and Heberle, J. and Offenhäusser,
A.},
title = {{S}canning {P}robe {M}icroscopic {S}tudies of the
{O}riented {A}ttachment and {M}embrane {R}econstitution of
{C}ytochrome c {O}xidase to a {G}old {E}lectrode},
journal = {Langmuir},
volume = {21},
issn = {0743-7463},
address = {Washington, DC},
publisher = {ACS Publ.},
reportid = {PreJuSER-47540},
pages = {8580 - 8583},
year = {2005},
note = {Record converted from VDB: 12.11.2012},
abstract = {Scanning probe microscopy was used to monitor the resulting
surface of the oriented incorporation of cytochrome c
oxidase into electrode supported lipid bilayer at four
crucial stages with molecular resolution. We were able to
reveal the formation of a densely packed monolayer of the
active ester dithio(succiniimidylepropionate) (DTSP) and the
covalent linkage of the nitrilotriacetic acid (NTA) to the
thiol anchored DTSP by scanning tunneling microscopy. Atomic
force microscopy investigations showed that the detergent
solubilized oxidase is immobilized as monomers and small
aggregates via histidine residues. Finally, the
reconstitution of the proteins within the supported membrane
was verified. The amount of oxidase immobilized within the
solid supported membrane was estimated.},
keywords = {Electrodes / Electron Transport Complex IV: chemistry /
Gold: chemistry / Membranes, Artificial / Microscopy,
Scanning Probe: methods / Particle Size / Sensitivity and
Specificity / Surface Properties / Membranes, Artificial
(NLM Chemicals) / Gold (NLM Chemicals) / Electron Transport
Complex IV (NLM Chemicals) / J (WoSType)},
cin = {CNI / ISG-2 / IBI-2},
ddc = {670},
cid = {I:(DE-Juel1)VDB381 / I:(DE-Juel1)VDB42 / I:(DE-Juel1)VDB58},
pnm = {Materialien, Prozesse und Bauelemente für die Mikro- und
Nanoelektronik / Neurowissenschaften},
pid = {G:(DE-Juel1)FUEK252 / G:(DE-Juel1)FUEK255},
shelfmark = {Chemistry, Multidisciplinary / Chemistry, Physical /
Materials Science, Multidisciplinary},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:16142929},
UT = {WOS:000231789800004},
doi = {10.1021/la051195x},
url = {https://juser.fz-juelich.de/record/47540},
}