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@ARTICLE{Enderlein:48349,
author = {Enderlein, J. and Gregor, I. and Patra, D. and Fitter, J.},
title = {{S}tatistical analysis of diffusion coefficient
determination by fluorescence correlation spectroscopy},
journal = {Journal of fluorescence},
volume = {15},
issn = {1053-0509},
address = {New York, NY},
publisher = {Springer Science + Business Media B.V.},
reportid = {PreJuSER-48349},
pages = {415 - 422},
year = {2005},
note = {Record converted from VDB: 12.11.2012},
abstract = {Fluorescence correlation spectroscopy (FCS) has become an
important and widely used technique for many applications in
physics, chemistry, and biology. The parameter most
frequently addressed by FCS is the diffusion of molecules in
solution. Due to the highly non-linear connection between
the diffusion coefficient and a measured autocorrelation
function, it is extremely difficult to analyse the accuracy
of the diffusion-coefficient determination in a FCS
experiment. Here, we present a simplified analysis based on
some general maximum-likelihood considerations, and
numerical result are given for the dependence of the
accuracy of the diffusion-coefficient determination on
sample concentration, brightness, and measurement time.
Optimal concentration values for performing FCS are found.},
keywords = {J (WoSType)},
cin = {IBI-1 / IBI-2},
ddc = {620},
cid = {I:(DE-Juel1)VDB57 / I:(DE-Juel1)VDB58},
pnm = {Neurowissenschaften},
pid = {G:(DE-Juel1)FUEK255},
shelfmark = {Biochemical Research Methods / Chemistry, Analytical},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:15986160},
UT = {WOS:000230175600015},
doi = {10.1007/s10895-005-2633-0},
url = {https://juser.fz-juelich.de/record/48349},
}