000048448 001__ 48448
000048448 005__ 20200610184459.0
000048448 0247_ $$2Handle$$a2128/456
000048448 0247_ $$2URI$$a456
000048448 037__ $$aPreJuSER-48448
000048448 0881_ $$aJuel-4186
000048448 088__ $$2JUEL$$aJuel-4186
000048448 1001_ $$0P:(DE-Juel1)VDB57503$$aCesa, Claudia Marina$$b0$$eCorresponding author$$uFZJ
000048448 245__ $$aMicrostructured elastomer films to measure dynamic traction forces of living animal cells with high spatial resolution. Establishment of the technique and first results on cardiac myocytes
000048448 260__ $$aJülich$$bForschungszentrum Jülich GmbH Zentralbibliothek, Verlag$$c2005
000048448 300__ $$a105 p.
000048448 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis
000048448 3367_ $$0PUB:(DE-HGF)3$$2PUB:(DE-HGF)$$aBook
000048448 3367_ $$02$$2EndNote$$aThesis
000048448 3367_ $$2DRIVER$$adoctoralThesis
000048448 3367_ $$2BibTeX$$aPHDTHESIS
000048448 3367_ $$2DataCite$$aOutput Types/Dissertation
000048448 3367_ $$2ORCID$$aDISSERTATION
000048448 4900_ $$0PERI:(DE-600)2414853-2$$822692$$aBerichte des Forschungszentrums Jülich$$v4186$$x0944-2952
000048448 502__ $$aBonn, Univ., Diss., 2005$$bDr. (Univ.)$$cUniv. Bonn$$d2005
000048448 500__ $$aRecord converted from VDB: 12.11.2012
000048448 520__ $$aAlmost all cell types are able to create mechanical forces. These forces are very important for many cell functions like cell adhesion, cell migration or cell division. Mechanical forces are produced in the cell mainly by the contraction of the actin-myosin complex. In adherent cells, the actin cytoskeleton anchors to the substrate via integrins. The linkage between integrin and the actin cytoskeleton is mediated by highly dynamic protein complexes, known as focal contacts. Thus, mechanical forces are transmitted to the substrates at the level of the focal contacts. In this project a technique for measuring mechanical forces transmitted at sites of focal adhesions based on (Balaban et al., 2001) was developed and adapted. In addition, as a first application, forces created by single beating myocytes were measured. Cells isolated from hearts of neonatal rat embryos were cultivated on fibronectin coated elastomers. Upon contraction of single beating cardiac myocytes, these substrates were reversibly deformed. Regular patterns of microstructures imprinted into the surface of the elastomer served as markers for the deformations. The micropattern was prepared by curing the elastomer in contact with a silicon master which exhibited the negative of the pattern of interest. The microstructure was prepared in a silicon dioxide layer having a thickness below 500 nm. Appropriate silicon dioxide coated silicon masters were prepared by adapting standard techniques from semiconductor technology. The mechanical properties of the used elastomer were carefully characterized. It was found that the material behaves as a linear and isotropic elastic medium. Mechanical forces exerted by cells to the substrates were measured by solving the inverse problem of elasticity theory (Schwarz et al., 2003; Schwarz et al., 2002). In this work, the force dipole tensor was introduced as measure of the mechanical activity of the whole cell. Cardiac myocytes were examined after one or two days in culture. Single beating cells with visible focal adhesions that deformed the microstructured substrates were selected for observation. Live cell imaging was performed using reflection interference contrast microscopy (RICM). In this way, the sites of focal adhesions and the microstructures could be localised by the same method. With this optimised technique, the effect of different stiffness of the substrates on force production (magnitude and transmission at the substrates) was also studied. Results on substrates of five different stiffnesses are shown and discussed.
000048448 536__ $$0G:(DE-Juel1)FUEK242$$2G:(DE-HGF)$$aKondensierte Materie$$cM02$$x0
000048448 655_7 $$aHochschulschrift$$xDissertation (Univ.)
000048448 8564_ $$uhttps://juser.fz-juelich.de/record/48448/files/J%C3%BCl_4186_Cesa.pdf$$yOpenAccess
000048448 909CO $$ooai:juser.fz-juelich.de:48448$$pdnbdelivery$$pVDB$$pdriver$$popen_access$$popenaire
000048448 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000048448 9141_ $$y2005
000048448 9131_ $$0G:(DE-Juel1)FUEK242$$bMaterie$$kM02$$lKondensierte Materie$$vKondensierte Materie$$x0
000048448 9201_ $$0I:(DE-Juel1)VDB44$$d31.12.2001$$gISG$$kISG-4$$lInstitut für biologisch-anorganische Grenzflächen$$x0
000048448 970__ $$aVDB:(DE-Juel1)76199
000048448 9801_ $$aFullTexts
000048448 980__ $$aVDB
000048448 980__ $$aJUWEL
000048448 980__ $$aConvertedRecord
000048448 980__ $$aphd
000048448 980__ $$aI:(DE-Juel1)ICS-7-20110106
000048448 980__ $$aUNRESTRICTED
000048448 980__ $$aFullTexts
000048448 981__ $$aI:(DE-Juel1)IBI-2-20200312
000048448 981__ $$aI:(DE-Juel1)ICS-7-20110106