| 001 | 48448 | ||
| 005 | 20200610184459.0 | ||
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| 088 | 1 | _ | |a Juel-4186 |
| 088 | _ | _ | |a Juel-4186 |2 JUEL |
| 100 | 1 | _ | |0 P:(DE-Juel1)VDB57503 |a Cesa, Claudia Marina |b 0 |e Corresponding author |u FZJ |
| 245 | _ | _ | |a Microstructured elastomer films to measure dynamic traction forces of living animal cells with high spatial resolution. Establishment of the technique and first results on cardiac myocytes |
| 260 | _ | _ | |a Jülich |b Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag |c 2005 |
| 300 | _ | _ | |a 105 p. |
| 336 | 7 | _ | |0 PUB:(DE-HGF)11 |2 PUB:(DE-HGF) |a Dissertation / PhD Thesis |
| 336 | 7 | _ | |0 PUB:(DE-HGF)3 |2 PUB:(DE-HGF) |a Book |
| 336 | 7 | _ | |0 2 |2 EndNote |a Thesis |
| 336 | 7 | _ | |2 DRIVER |a doctoralThesis |
| 336 | 7 | _ | |2 BibTeX |a PHDTHESIS |
| 336 | 7 | _ | |2 DataCite |a Output Types/Dissertation |
| 336 | 7 | _ | |2 ORCID |a DISSERTATION |
| 490 | 0 | _ | |0 PERI:(DE-600)2414853-2 |8 22692 |a Berichte des Forschungszentrums Jülich |v 4186 |x 0944-2952 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 502 | _ | _ | |a Bonn, Univ., Diss., 2005 |b Dr. (Univ.) |c Univ. Bonn |d 2005 |
| 520 | _ | _ | |a Almost all cell types are able to create mechanical forces. These forces are very important for many cell functions like cell adhesion, cell migration or cell division. Mechanical forces are produced in the cell mainly by the contraction of the actin-myosin complex. In adherent cells, the actin cytoskeleton anchors to the substrate via integrins. The linkage between integrin and the actin cytoskeleton is mediated by highly dynamic protein complexes, known as focal contacts. Thus, mechanical forces are transmitted to the substrates at the level of the focal contacts. In this project a technique for measuring mechanical forces transmitted at sites of focal adhesions based on (Balaban et al., 2001) was developed and adapted. In addition, as a first application, forces created by single beating myocytes were measured. Cells isolated from hearts of neonatal rat embryos were cultivated on fibronectin coated elastomers. Upon contraction of single beating cardiac myocytes, these substrates were reversibly deformed. Regular patterns of microstructures imprinted into the surface of the elastomer served as markers for the deformations. The micropattern was prepared by curing the elastomer in contact with a silicon master which exhibited the negative of the pattern of interest. The microstructure was prepared in a silicon dioxide layer having a thickness below 500 nm. Appropriate silicon dioxide coated silicon masters were prepared by adapting standard techniques from semiconductor technology. The mechanical properties of the used elastomer were carefully characterized. It was found that the material behaves as a linear and isotropic elastic medium. Mechanical forces exerted by cells to the substrates were measured by solving the inverse problem of elasticity theory (Schwarz et al., 2003; Schwarz et al., 2002). In this work, the force dipole tensor was introduced as measure of the mechanical activity of the whole cell. Cardiac myocytes were examined after one or two days in culture. Single beating cells with visible focal adhesions that deformed the microstructured substrates were selected for observation. Live cell imaging was performed using reflection interference contrast microscopy (RICM). In this way, the sites of focal adhesions and the microstructures could be localised by the same method. With this optimised technique, the effect of different stiffness of the substrates on force production (magnitude and transmission at the substrates) was also studied. Results on substrates of five different stiffnesses are shown and discussed. |
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| 655 | _ | 7 | |a Hochschulschrift |x Dissertation (Univ.) |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/48448/files/J%C3%BCl_4186_Cesa.pdf |y OpenAccess |
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| 914 | 1 | _ | |y 2005 |
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| 920 | 1 | _ | |0 I:(DE-Juel1)VDB44 |d 31.12.2001 |g ISG |k ISG-4 |l Institut für biologisch-anorganische Grenzflächen |x 0 |
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