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000049006 0247_ $$2pmid$$apmid:15919078
000049006 0247_ $$2DOI$$a10.1016/j.febslet.2005.05.010
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000049006 041__ $$aeng
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000049006 084__ $$2WoS$$aBiochemistry & Molecular Biology
000049006 084__ $$2WoS$$aBiophysics
000049006 084__ $$2WoS$$aCell Biology
000049006 1001_ $$0P:(DE-Juel1)VDB58062$$aMironova, O. S.$$b0$$uFZJ
000049006 245__ $$aFunctional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli
000049006 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2005
000049006 300__ $$a3147 - 3151
000049006 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000049006 440_0 $$02052$$aFEBS Letters$$v579$$x0014-5793$$y14
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000049006 520__ $$aSensory rhodopsin II (SRII) from Halobacterium salinarum is heterologously expressed in Escherichia coli with a yield of 3-4 mg of purified SRII per liter cell culture. UV/Vis absorption spectroscopy display bands characteristic for native SRII. The resonance Raman spectrum provides evidence for a strongly hydrogen-bonded Schiff base like in mammalian rhodopsin but unlike to the homologous pSRII from Natronobacterium pharaonis. Laser flash spectroscopy indicates that SRII in detergent as well as after reconstitution into polar lipids shows its typical photochemical properties with prolonged photocycle kinetics. The first functional heterologous expression of SRII from H. salinarum provides the basis for studies with its cognate transducer HtrII to investigate the molecular processes involved in phototransduction as well as in chemotransduction.
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000049006 650_2 $$2MeSH$$aElectrophoresis
000049006 650_2 $$2MeSH$$aEscherichia coli: genetics
000049006 650_2 $$2MeSH$$aHalobacterium salinarum: genetics
000049006 650_2 $$2MeSH$$aKinetics
000049006 650_2 $$2MeSH$$aRecombinant Proteins: genetics
000049006 650_2 $$2MeSH$$aRecombinant Proteins: isolation & purification
000049006 650_2 $$2MeSH$$aRecombinant Proteins: metabolism
000049006 650_2 $$2MeSH$$aSensory Rhodopsins: genetics
000049006 650_2 $$2MeSH$$aSensory Rhodopsins: isolation & purification
000049006 650_2 $$2MeSH$$aSensory Rhodopsins: metabolism
000049006 650_2 $$2MeSH$$aSpectrum Analysis, Raman
000049006 650_7 $$00$$2NLM Chemicals$$aRecombinant Proteins
000049006 650_7 $$00$$2NLM Chemicals$$aSensory Rhodopsins
000049006 650_7 $$2WoSType$$aJ
000049006 65320 $$2Author$$aphotosensory receptor
000049006 65320 $$2Author$$aarchaeal phototaxis
000049006 65320 $$2Author$$aphotocycle
000049006 65320 $$2Author$$amembrane protein
000049006 65320 $$2Author$$aUV-Vis spectroscopy
000049006 65320 $$2Author$$aresonance Raman spectroscopy
000049006 7001_ $$0P:(DE-Juel1)VDB4647$$aEfremov, R. N.$$b1$$uFZJ
000049006 7001_ $$0P:(DE-Juel1)VDB58064$$aPerson, B.$$b2$$uFZJ
000049006 7001_ $$0P:(DE-Juel1)VDB572$$aHeberle, J.$$b3$$uFZJ
000049006 7001_ $$0P:(DE-HGF)0$$aBudyak, I. L.$$b4
000049006 7001_ $$0P:(DE-Juel1)131957$$aBüldt, G.$$b5$$uFZJ
000049006 7001_ $$0P:(DE-Juel1)VDB1421$$aSchlesinger, R.$$b6$$uFZJ
000049006 773__ $$0PERI:(DE-600)1460391-3$$a10.1016/j.febslet.2005.05.010$$gVol. 579, p. 3147 - 3151$$p3147 - 3151$$q579<3147 - 3151$$tFEBS letters$$v579$$x0014-5793$$y2005
000049006 8567_ $$uhttp://dx.doi.org/10.1016/j.febslet.2005.05.010
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