TY  - JOUR
AU  - Mironova, O. S.
AU  - Efremov, R. N.
AU  - Person, B.
AU  - Heberle, J.
AU  - Budyak, I. L.
AU  - Büldt, G.
AU  - Schlesinger, R.
TI  - Functional characterization of sensory rhodopsin II from Halobacterium salinarum expressed in Escherichia coli
JO  - FEBS letters
VL  - 579
SN  - 0014-5793
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PreJuSER-49006
SP  - 3147 - 3151
PY  - 2005
N1  - Record converted from VDB: 12.11.2012
AB  - Sensory rhodopsin II (SRII) from Halobacterium salinarum is heterologously expressed in Escherichia coli with a yield of 3-4 mg of purified SRII per liter cell culture. UV/Vis absorption spectroscopy display bands characteristic for native SRII. The resonance Raman spectrum provides evidence for a strongly hydrogen-bonded Schiff base like in mammalian rhodopsin but unlike to the homologous pSRII from Natronobacterium pharaonis. Laser flash spectroscopy indicates that SRII in detergent as well as after reconstitution into polar lipids shows its typical photochemical properties with prolonged photocycle kinetics. The first functional heterologous expression of SRII from H. salinarum provides the basis for studies with its cognate transducer HtrII to investigate the molecular processes involved in phototransduction as well as in chemotransduction.
KW  - Electrophoresis
KW  - Escherichia coli: genetics
KW  - Halobacterium salinarum: genetics
KW  - Kinetics
KW  - Recombinant Proteins: genetics
KW  - Recombinant Proteins: isolation & purification
KW  - Recombinant Proteins: metabolism
KW  - Sensory Rhodopsins: genetics
KW  - Sensory Rhodopsins: isolation & purification
KW  - Sensory Rhodopsins: metabolism
KW  - Spectrum Analysis, Raman
KW  - Recombinant Proteins (NLM Chemicals)
KW  - Sensory Rhodopsins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15919078
UR  - <Go to ISI:>//WOS:000229822500031
DO  - DOI:10.1016/j.febslet.2005.05.010
UR  - https://juser.fz-juelich.de/record/49006
ER  -