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@ARTICLE{Mironova:49006,
      author       = {Mironova, O. S. and Efremov, R. N. and Person, B. and
                      Heberle, J. and Budyak, I. L. and Büldt, G. and
                      Schlesinger, R.},
      title        = {{F}unctional characterization of sensory rhodopsin {II}
                      from {H}alobacterium salinarum expressed in {E}scherichia
                      coli},
      journal      = {FEBS letters},
      volume       = {579},
      issn         = {0014-5793},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {PreJuSER-49006},
      pages        = {3147 - 3151},
      year         = {2005},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Sensory rhodopsin II (SRII) from Halobacterium salinarum is
                      heterologously expressed in Escherichia coli with a yield of
                      3-4 mg of purified SRII per liter cell culture. UV/Vis
                      absorption spectroscopy display bands characteristic for
                      native SRII. The resonance Raman spectrum provides evidence
                      for a strongly hydrogen-bonded Schiff base like in mammalian
                      rhodopsin but unlike to the homologous pSRII from
                      Natronobacterium pharaonis. Laser flash spectroscopy
                      indicates that SRII in detergent as well as after
                      reconstitution into polar lipids shows its typical
                      photochemical properties with prolonged photocycle kinetics.
                      The first functional heterologous expression of SRII from H.
                      salinarum provides the basis for studies with its cognate
                      transducer HtrII to investigate the molecular processes
                      involved in phototransduction as well as in
                      chemotransduction.},
      keywords     = {Electrophoresis / Escherichia coli: genetics /
                      Halobacterium salinarum: genetics / Kinetics / Recombinant
                      Proteins: genetics / Recombinant Proteins: isolation $\&$
                      purification / Recombinant Proteins: metabolism / Sensory
                      Rhodopsins: genetics / Sensory Rhodopsins: isolation $\&$
                      purification / Sensory Rhodopsins: metabolism / Spectrum
                      Analysis, Raman / Recombinant Proteins (NLM Chemicals) /
                      Sensory Rhodopsins (NLM Chemicals) / J (WoSType)},
      cin          = {IBI-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB58},
      pnm          = {Neurowissenschaften},
      pid          = {G:(DE-Juel1)FUEK255},
      shelfmark    = {Biochemistry $\&$ Molecular Biology / Biophysics / Cell
                      Biology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:15919078},
      UT           = {WOS:000229822500031},
      doi          = {10.1016/j.febslet.2005.05.010},
      url          = {https://juser.fz-juelich.de/record/49006},
}