Home > Publications database > One step ahead: Role of filopodia in adhesion formation during cell migration of keratinocytes > print

001     4909
005     20200402205620.0
024 7 _ |2 pmid
|a pmid:19100734
024 7 _ |2 DOI
|a 10.1016/j.yexcr.2008.11.008
024 7 _ |2 WOS
|a WOS:000265126900012
037 _ _ |a PreJuSER-4909
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Oncology
084 _ _ |2 WoS
|a Cell Biology
100 1 _ |a Schäfer, C.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB8500
245 _ _ |a One step ahead: Role of filopodia in adhesion formation during cell migration of keratinocytes
260 _ _ |a Orlando, Fla.
|b Academic Press
|c 2009
300 _ _ |a 1212 - 1224
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Experimental Cell Research
|x 0014-4827
|0 13781
|y 7
|v 315
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Cell adhesion is an essential prerequisite for cell function and movement. It depends strongly on focal adhesion complexes connecting the extracellular matrix to the actin cytoskeleton. Especially in moving cells focal adhesions are highly dynamic and believed to be formed closely behind the leading edge. Filopodia were thought to act mainly as guiding cues using their tip complexes for elongation. Here we show for keratinocytes a strong dependence of lamellipodial adhesion sites on filopodia. Upon stable contact of the VASP-containing tip spot to the substrate, a filopodial focal complex (filopodial FX) is formed right behind along the filopodia axis. These filopodial FXs are fully assembled, yet small adhesions containing all adhesion markers tested. Filopodial FXs when reached by the lamellipodium are just increased in size resulting in classical focal adhesions. At the same time most filopodia regain their elongation ability. Blocking filopodia inhibits development of new focal adhesions in the lamellipodium, while focal adhesion maturation in terms of vinculin exchange dynamics remains active. Our data therefore argue for a strong spatial and temporal dependence of focal adhesions on filopodial focal complexes in keratinocytes with filopodia not permanently initiated via new clustering of actin filaments to induce elongation.
536 _ _ |a Kondensierte Materie
|c P54
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK414
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Cell Adhesion: physiology
650 _ 2 |2 MeSH
|a Cell Adhesion Molecules: metabolism
650 _ 2 |2 MeSH
|a Cell Movement: physiology
650 _ 2 |2 MeSH
|a Cells, Cultured
650 _ 2 |2 MeSH
|a Cytoskeletal Proteins: genetics
650 _ 2 |2 MeSH
|a Cytoskeletal Proteins: metabolism
650 _ 2 |2 MeSH
|a Fluorescence Recovery After Photobleaching
650 _ 2 |2 MeSH
|a Focal Adhesions: metabolism
650 _ 2 |2 MeSH
|a Glycoproteins: genetics
650 _ 2 |2 MeSH
|a Glycoproteins: metabolism
650 _ 2 |2 MeSH
|a Humans
650 _ 2 |2 MeSH
|a Keratinocytes: cytology
650 _ 2 |2 MeSH
|a Keratinocytes: physiology
650 _ 2 |2 MeSH
|a Microfilament Proteins: metabolism
650 _ 2 |2 MeSH
|a Paxillin: genetics
650 _ 2 |2 MeSH
|a Paxillin: metabolism
650 _ 2 |2 MeSH
|a Phosphoproteins: metabolism
650 _ 2 |2 MeSH
|a Pseudopodia: metabolism
650 _ 2 |2 MeSH
|a Pseudopodia: ultrastructure
650 _ 2 |2 MeSH
|a Recombinant Fusion Proteins: genetics
650 _ 2 |2 MeSH
|a Recombinant Fusion Proteins: metabolism
650 _ 2 |2 MeSH
|a Talin: genetics
650 _ 2 |2 MeSH
|a Talin: metabolism
650 _ 2 |2 MeSH
|a Vinculin: genetics
650 _ 2 |2 MeSH
|a Vinculin: metabolism
650 _ 2 |2 MeSH
|a Zyxin
650 _ 7 |0 0
|2 NLM Chemicals
|a Cell Adhesion Molecules
650 _ 7 |0 0
|2 NLM Chemicals
|a Cytoskeletal Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Glycoproteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Microfilament Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Paxillin
650 _ 7 |0 0
|2 NLM Chemicals
|a Phosphoproteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Recombinant Fusion Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Talin
650 _ 7 |0 0
|2 NLM Chemicals
|a ZYX protein, human
650 _ 7 |0 0
|2 NLM Chemicals
|a Zyxin
650 _ 7 |0 0
|2 NLM Chemicals
|a vasodilator-stimulated phosphoprotein
650 _ 7 |0 125361-02-6
|2 NLM Chemicals
|a Vinculin
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a Filopodium
653 2 0 |2 Author
|a Cell migration
653 2 0 |2 Author
|a VASP
653 2 0 |2 Author
|a Cell adhesion
653 2 0 |2 Author
|a Focal adhesion
653 2 0 |2 Author
|a Tip complex
653 2 0 |2 Author
|a Keratinocytes
653 2 0 |2 Author
|a Actin
653 2 0 |2 Author
|a Fascin
700 1 _ |a Borm, B.
|b 1
|0 P:(DE-HGF)0
700 1 _ |a Born, S.
|b 2
|u FZJ
|0 P:(DE-Juel1)161241
700 1 _ |a Möhl, C.
|b 3
|u FZJ
|0 P:(DE-Juel1)VDB71075
700 1 _ |a Eibl, E.M.
|b 4
|u FZJ
|0 P:(DE-Juel1)VDB84707
700 1 _ |a Hoffmann, B.
|b 5
|u FZJ
|0 P:(DE-Juel1)VDB27696
773 _ _ |a 10.1016/j.yexcr.2008.11.008
|g Vol. 315, p. 1212 - 1224
|p 1212 - 1224
|q 315<1212 - 1224
|0 PERI:(DE-600)1466780-0
|t Experimental cell research
|v 315
|y 2009
|x 0014-4827
856 7 _ |u http://dx.doi.org/10.1016/j.yexcr.2008.11.008
909 C O |o oai:juser.fz-juelich.de:4909
|p VDB
913 1 _ |k P54
|v Kondensierte Materie
|l Kondensierte Materie
|b Materie
|z entfällt bis 2009
|0 G:(DE-Juel1)FUEK414
|x 0
914 1 _ |y 2009
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k IBN-4
|l Biomechanik
|d 31.12.2010
|g IBN
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|x 0
970 _ _ |a VDB:(DE-Juel1)112243
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980 _ _ |a ConvertedRecord
980 _ _ |a journal
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980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBI-2-20200312
981 _ _ |a I:(DE-Juel1)ICS-7-20110106

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