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@ARTICLE{Bauer:49418,
author = {Bauer, F. and Schweimer, K. and Meiselbach, H. and
Hoffmann, S. and Rosch, P. and Sticht, H.},
title = {{S}tructural characterization of {L}yn-{SH}3 domain in
complex with a herpesviral protein reveals an extended
recognition motif that enhances binding affinity},
journal = {Protein science},
volume = {14},
issn = {0961-8368},
address = {Hoboken, NJ},
publisher = {Wiley},
reportid = {PreJuSER-49418},
pages = {2487 -2498},
year = {2005},
note = {Record converted from VDB: 12.11.2012},
abstract = {The Src homology 3 (SH3) domain of the Src family kinase
Lyn binds to the herpesviral tyrosine kinase interacting
protein (Tip) more than one order of magnitude stronger than
other closely related members of the Src family. In order to
identify the molecular basis for high-affinity binding, the
structure of free and Tip-bound Lyn-SH3 was determined by
NMR spectroscopy. Tip forms additional contacts outside its
classical proline-rich recognition motif and, in particular,
a strictly conserved leucine (L186) of the C-terminally
adjacent sequence stretch packs into a hydrophobic pocket on
the Lyn surface. Although the existence of this pocket is no
unique property of Lyn-SH3, Lyn is the only Src family
kinase that contains an additional aromatic residue (H41) in
the n-Src loop as part of this pocket. H41 covers L186 of
Tip by forming tight hydrophobic contacts, and model
calculations suggest that the increase in binding affinity
compared with other SH3 domains can mainly be attributed to
these additional interactions. These findings indicate that
this pocket can mediate specificity even between otherwise
closely related SH3 domains.},
keywords = {Models, Molecular / Multiprotein Complexes: chemistry /
Multiprotein Complexes: metabolism / Nuclear Magnetic
Resonance, Biomolecular: methods / Phosphoproteins:
chemistry / Phosphoproteins: metabolism / Protein Binding /
Protein Structure, Quaternary / Viral Proteins: chemistry /
Viral Proteins: metabolism / src Homology Domains /
src-Family Kinases: chemistry / src-Family Kinases:
metabolism / Multiprotein Complexes (NLM Chemicals) /
Phosphoproteins (NLM Chemicals) / Viral Proteins (NLM
Chemicals) / tyrosine kinase interacting protein, Saimiriine
herpesvirus 2 (NLM Chemicals) / lyn protein-tyrosine kinase
(NLM Chemicals) / src-Family Kinases (NLM Chemicals) / J
(WoSType)},
cin = {IBI-2},
ddc = {610},
cid = {I:(DE-Juel1)VDB58},
pnm = {Neurowissenschaften},
pid = {G:(DE-Juel1)FUEK255},
shelfmark = {Biochemistry $\&$ Molecular Biology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:16155203},
pmc = {pmc:PMC2253286},
UT = {WOS:000232233400001},
doi = {10.1110/ps.051563605},
url = {https://juser.fz-juelich.de/record/49418},
}